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In adults drug transfer from blood into brain is mostly restricted by efflux mechanisms (ATP-binding cassette, ABC transporters). These mechanisms have been little studied during brain development. Here expression of eight ABC transporters (<jats:italic>abcb1a<\/jats:italic>, <jats:italic>abcb1b<\/jats:italic>, <jats:italic>abcg2<\/jats:italic>, <jats:italic>abcc1<\/jats:italic>, <jats:italic>abcc2<\/jats:italic>, <jats:italic>abcc3<\/jats:italic>, <jats:italic>abcc4<\/jats:italic>, <jats:italic>abcc5<\/jats:italic>) and activity of conjugating enzyme glutathione-s-transferase (GST) were measured in livers, brain cortices (blood-brain-barrier) and choroid plexuses (blood-cerebrospinal fluid, CSF, barrier) during postnatal rat development. Controls were compared to animals chronically injected (4 days, 200\u2009mg\/kg\/day) with known <jats:italic>abcb1a<\/jats:italic> inducer diallyl sulfide (DAS). Results reveal both tissue- and age-dependent regulation. In liver <jats:italic>abcb1a<\/jats:italic> and <jats:italic>abcc3<\/jats:italic> were up-regulated at all ages. In cortex <jats:italic>abcb1a\/b<\/jats:italic>, <jats:italic>abcg2<\/jats:italic> and <jats:italic>abcc4\/abcc5<\/jats:italic> were up-regulated in adults only, while in choroid plexus <jats:italic>abcb1a<\/jats:italic> and <jats:italic>abcc2<\/jats:italic> were up-regulated only at P14. DAS treatment increased GST activity in livers, but not in cortex or choroid plexuses. Immunocytochemistry of ABC transporters at the CSF-brain interface showed that PGP and BCRP predominated in neuroepithelium while MRP2\/4\/5 were prominent in adult ependyma. These results indicate an age-related capacity of brain barriers to dynamically regulate their defence mechanisms when chronically challenged by xenobiotic compounds.<\/jats:p>","DOI":"10.1038\/s41598-019-42402-8","type":"journal-article","created":{"date-parts":[[2019,4,12]],"date-time":"2019-04-12T10:02:59Z","timestamp":1555063379000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Developmental differences in the expression of ABC transporters at rat brain barrier interfaces following chronic exposure to diallyl sulfide"],"prefix":"10.1038","volume":"9","author":[{"given":"Liam M.","family":"Koehn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna M.","family":"Dziegielewska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6645-8626","authenticated-orcid":false,"given":"Kjeld","family":"M\u00f8llg\u00e5rd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elodie","family":"Saudrais","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Strazielle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Francois","family":"Ghersi-Egea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6660-7639","authenticated-orcid":false,"given":"Norman R.","family":"Saunders","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark D.","family":"Habgood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,4,12]]},"reference":[{"key":"42402_CR1","doi-asserted-by":"publisher","first-page":"885","DOI":"10.2165\/00002018-200225120-00006","volume":"25","author":"K Uhl","year":"2002","unstructured":"Uhl, K., Kennedy, D. 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