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Here, we identified CteG as the first <jats:italic><jats:underline>C<\/jats:underline><\/jats:italic>. <jats:italic><jats:underline>t<\/jats:underline><\/jats:italic><jats:italic>rachomatis<\/jats:italic><jats:underline>e<\/jats:underline>ffector associated with the <jats:underline>G<\/jats:underline>olgi. For this, <jats:italic>C<\/jats:italic>. <jats:italic>trachomatis<\/jats:italic> strains expressing candidate effectors fused to a double hemagglutinin (2HA) tag were constructed. Then, among these strains, immunofluorescence microscopy revealed that CteG-2HA was delivered into the cytoplasm of infected cells. Between 16\u201320\u2009h post-infection, CteG-2HA mostly associated with the Golgi; however, CteG-2HA also appeared at the host cell plasma membrane, and at 30 or 40\u2009h post-infection this was its predominant localization. This change in the main localization of CteG-2HA was independent of intact microfilaments or microtubules. Ectopic expression of different regions of CteG (656 amino acid residues) in uninfected cells revealed that its first 100 residues contain a Golgi targeting region. Although a <jats:italic>C<\/jats:italic>. <jats:italic>trachomatis cteG<\/jats:italic> mutant did not display a defect in intracellular multiplication, CteG induced a vacuolar protein sorting defect when expressed in <jats:italic>Saccharomyces cerevisiae<\/jats:italic>. This suggested that CteG might function by subverting host cell vesicular transport.<\/jats:p>","DOI":"10.1038\/s41598-019-42647-3","type":"journal-article","created":{"date-parts":[[2019,4,16]],"date-time":"2019-04-16T10:03:21Z","timestamp":1555409001000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["CteG is a Chlamydia trachomatis effector protein that associates with the Golgi complex of infected host cells"],"prefix":"10.1038","volume":"9","author":[{"given":"Sara V.","family":"Pais","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte E.","family":"Key","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3767-2209","authenticated-orcid":false,"given":"V\u00edtor","family":"Borges","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6650-7371","authenticated-orcid":false,"given":"In\u00eas S.","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2697-2399","authenticated-orcid":false,"given":"Jo\u00e3o Paulo","family":"Gomes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1663-8389","authenticated-orcid":false,"given":"Derek J.","family":"Fisher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5592-8397","authenticated-orcid":false,"given":"Lu\u00eds Jaime","family":"Mota","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,4,16]]},"reference":[{"key":"42647_CR1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)62182-0","author":"HR Taylor","year":"2014","unstructured":"Taylor, H. 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