{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T00:57:47Z","timestamp":1772499467120,"version":"3.50.1"},"reference-count":51,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2019,4,18]],"date-time":"2019-04-18T00:00:00Z","timestamp":1555545600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2019,4,18]],"date-time":"2019-04-18T00:00:00Z","timestamp":1555545600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Visceral leishmaniasis is a chronic disease that affects humans and dogs as well. Dogs, the domestic reservoir of <jats:italic>Leishmania<\/jats:italic>, play a central role in the transmission of visceral leishmaniasis, the most severe form of this disease. Neutrophils are the most abundant leukocytes in blood and interact with the parasite after infection. Here, we evaluate the effector properties of neutrophils from healthy and naturally <jats:italic>Leishmania infantum-<\/jats:italic>infected dogs. Our results showed that the parasite induced neutrophil extracellular trap (NET) release from neutrophils in both groups. Additionally, phagocytosis and NETs contributed differently to parasite killing by neutrophils from healthy and infected animals, and IFN-\u03b3, IL-8, IL-4 and TNF-\u03b1 production by neutrophils from both groups were differentially modulated by the parasite. Our results contribute to a better understanding of the complex role played by neutrophils in canine visceral leishmaniasis, which may favor the development of more effective therapies.<\/jats:p>","DOI":"10.1038\/s41598-019-42687-9","type":"journal-article","created":{"date-parts":[[2019,4,18]],"date-time":"2019-04-18T10:08:13Z","timestamp":1555582093000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Neutrophil properties in healthy and Leishmania infantum-naturally infected dogs"],"prefix":"10.1038","volume":"9","author":[{"given":"Amanda Brito","family":"Wardini","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucia Helena","family":"Pinto-da-Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia Rocha","family":"Nadaes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle Tanny","family":"Nascimento","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno Mendes","family":"Roatt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandre Barbosa","family":"Reis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelvinson Fernandes","family":"Viana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodolfo Cordeiro","family":"Giunchetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elvira Maria","family":"Saraiva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,4,18]]},"reference":[{"key":"42687_CR1","doi-asserted-by":"publisher","first-page":"9375","DOI":"10.1073\/pnas.0703678104","volume":"104","author":"J Lukes","year":"2007","unstructured":"Lukes, J. et al. Evolutionary and geographical history of the Leishmania donovani complex with a revision of current taxonomy. Proc. Natl. Acad. Sci. USA 104, 9375\u201380 (2007).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"42687_CR2","doi-asserted-by":"publisher","first-page":"e2225","DOI":"10.1371\/journal.pntd.0002225","volume":"7","author":"V Foglia Manzillo","year":"2013","unstructured":"Foglia Manzillo, V. et al. Prospective study on the incidence and progression of clinical signs in na\u00efve dogs naturally infected by Leishmania infantum. PLoS Negl Trop Dis. 7, e2225 (2013).","journal-title":"PLoS Negl Trop Dis."},{"key":"42687_CR3","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1111\/j.1365-3024.2006.00840.x","volume":"28","author":"CL Barbi\u00e9ri","year":"2006","unstructured":"Barbi\u00e9ri, C. L. Immunology of canine leishmaniasis. 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