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Recently, the RNA-binding protein Musashi-1 (Msi1), which mediates the maintenance and self-renewal of stem cells and acts as a translational regulator, has been associated with promoting ZIKV replication, neurotropism, and pathology. Msi1 predominantly binds to single-stranded motifs in the 3\u2032 untranslated region (UTR) of RNA that contain a\n                    <jats:italic>UAG<\/jats:italic>\n                    trinucleotide in their core. We systematically analyzed the properties of Musashi binding elements (MBEs) in the 3\u2032UTR of flaviviruses with a thermodynamic model for RNA folding. Our results indicate that MBEs in ZIKV 3\u2032UTRs occur predominantly in unpaired, single-stranded structural context, thus corroborating experimental observations by a biophysical model of RNA structure formation. Statistical analysis and comparison with related viruses show that ZIKV MBEs are maximally accessible among mosquito-borne flaviviruses. Our study addresses the broader question of whether other emerging arboviruses can cause similar neurotropic effects through the same mechanism in the developing fetus by establishing a link between the biophysical properties of viral RNA and teratogenicity. Moreover, our thermodynamic model can explain recent experimental findings and predict the Msi1-related neurotropic potential of other viruses.\n                  <\/jats:p>","DOI":"10.1038\/s41598-019-43390-5","type":"journal-article","created":{"date-parts":[[2019,5,6]],"date-time":"2019-05-06T20:21:22Z","timestamp":1557174082000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Musashi binding elements in Zika and related Flavivirus 3\u2032UTRs: A comparative study in silico"],"prefix":"10.1038","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7487-266X","authenticated-orcid":false,"given":"Adriano de Bernardi","family":"Schneider","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0925-5205","authenticated-orcid":false,"given":"Michael T.","family":"Wolfinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,5,6]]},"reference":[{"key":"43390_CR1","doi-asserted-by":"publisher","first-page":"665","DOI":"10.1007\/s00705-015-2695-5","volume":"161","author":"J Tognarelli","year":"2016","unstructured":"Tognarelli, J. et al. 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