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The analysis of the content and the surface of microvesicles allow conclusions about the cells they are originating from and the underlying pathology. Therefore, CSF microvesicles have been suggested to be promising targets to monitor the (etio)pathology of neurodegenerative diseases. Microvesicles in the CSF of 15 patients with Alzheimer\u2019s disease and 15 controls were analyzed by flow cytometry regarding the levels of CD3, CD4, CD45, CD64, BACE1, A\u03b2, APP and tau. The results were replicated in a second cohort comprising 14 patients with Alzheimer\u2019s disease and 9 controls. The levels of tau and APP were\u00a0reduced in microvesicles of Alzheimer\u2019s disease patients. A significant change was neither observed in the number of microvesicles nor in the expression of the other antigens. Tau and APP in microvesicles separated patients with Alzheimer\u2019s disease from controls with an AUC of 0.84 and 0.89 respectively. We conclude that tau and APP in CSF microvesicles are promising biomarkers which could directly provide information about the\u00a0Alzheimer pathology on a cellular level.<\/jats:p>","DOI":"10.1038\/s41598-019-43607-7","type":"journal-article","created":{"date-parts":[[2019,5,9]],"date-time":"2019-05-09T15:35:48Z","timestamp":1557416148000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Microvesicles from cerebrospinal fluid of patients with Alzheimer\u2019s disease display reduced concentrations of tau and APP protein"],"prefix":"10.1038","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9555-605X","authenticated-orcid":false,"given":"Philipp","family":"Spitzer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda-Marie","family":"Mulzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timo Jan","family":"Oberstein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5395-804X","authenticated-orcid":false,"given":"Luis Enrique","family":"Munoz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"Lewczuk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Kornhuber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0258-2484","authenticated-orcid":false,"given":"Martin","family":"Herrmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Manuel","family":"Maler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,5,8]]},"reference":[{"key":"43607_CR1","doi-asserted-by":"publisher","first-page":"276","DOI":"10.3389\/fnmol.2017.00276","volume":"10","author":"F Ciregia","year":"2017","unstructured":"Ciregia, F., Urbani, A. & Palmisano, G. 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