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In this work, one of the resulting mutants was characterized. This strain, selected with allyl alcohol and designated as the AA mutant, shows a dominant production of acids, a severely diminished butanol synthesis capacity, and produces acetone instead of isopropanol. Interestingly, this solvent-deficient strain was also found to have a limited consumption of two carbohydrates and to be still able to form spores, highlighting its particular phenotype. Sequencing of the AA mutant revealed point mutations in several genes including CIBE_0767 (\n                    <jats:italic>sigL<\/jats:italic>\n                    ), which encodes the \u03c3\n                    <jats:sup>54<\/jats:sup>\n                    sigma factor. Complementation with wild-type\n                    <jats:italic>sigL<\/jats:italic>\n                    fully restored solvent production and sugar assimilation and RT-qPCR analyses revealed its transcriptional control of several genes related to solventogensis, demonstrating the central role of \u03c3\n                    <jats:sup>54<\/jats:sup>\n                    in\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>beijerinckii<\/jats:italic>\n                    DSM 6423. Comparative genomics analysis suggested that this function is conserved at the species level, and this hypothesis was further confirmed through the deletion of\n                    <jats:italic>sigL<\/jats:italic>\n                    in the model strain\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>beijerinckii<\/jats:italic>\n                    NCIMB 8052.\n                  <\/jats:p>","DOI":"10.1038\/s41598-019-43822-2","type":"journal-article","created":{"date-parts":[[2019,5,10]],"date-time":"2019-05-10T06:04:37Z","timestamp":1557468277000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["\u03c354 (\u03c3L) plays a central role in carbon metabolism in the industrially relevant Clostridium beijerinckii"],"prefix":"10.1038","volume":"9","author":[{"given":"R\u00e9mi","family":"Hocq","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxime","family":"Bouilloux-Lafont","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Lopes Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Wasels","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,5,10]]},"reference":[{"key":"43822_CR1","unstructured":"IHS Chemical. 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