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Identification of novel vaginal <jats:italic>Lactobacillus<\/jats:italic> isolates that exhibit efficient colonisation and secrete anti-<jats:italic>Candida<\/jats:italic> factors is a promising strategy to prevent vulvovaginal candidiasis. The azole antifungal agents used to treat vulvovaginal candidiasis elicit adverse effects such as allergic responses and exhibit drug interactions. <jats:italic>Candida<\/jats:italic> strains with resistance to antifungal treatments are often reported. In this study, we isolated <jats:italic>Lactobacillus<\/jats:italic> species from healthy Korean women and investigated their antifungal effects against <jats:italic>C<\/jats:italic>. <jats:italic>albicans in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>. <jats:italic>Lactobacillus<\/jats:italic> conditioned supernatant (LCS) of <jats:italic>L<\/jats:italic>. <jats:italic>crispatus<\/jats:italic> and <jats:italic>L<\/jats:italic>. <jats:italic>fermentum<\/jats:italic> inhibited <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic> growth <jats:italic>in vitro<\/jats:italic>. A <jats:italic>Lactobacillus<\/jats:italic>-derived compound, which was not affected by proteolytic enzyme digestion and heat inactivation, inhibited growth and hyphal induction of <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic> after adjustment to neutral pH. Combination treatment with neutral LCSs of <jats:italic>L<\/jats:italic>. <jats:italic>crispatus<\/jats:italic> and <jats:italic>L<\/jats:italic>. <jats:italic>fermentum<\/jats:italic> effectively inhibited propagation of <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic> in a murine <jats:italic>in vivo<\/jats:italic> model of vulvovaginal candidiasis.<\/jats:p>","DOI":"10.1038\/s41598-019-44579-4","type":"journal-article","created":{"date-parts":[[2019,5,31]],"date-time":"2019-05-31T10:02:51Z","timestamp":1559296971000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":104,"title":["Vaginal lactobacilli inhibit growth and hyphae formation of Candida albicans"],"prefix":"10.1038","volume":"9","author":[{"given":"Sung Jae","family":"Jang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyeongju","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bomi","family":"Kwon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun Ju","family":"You","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"GwangPyo","family":"Ko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,5,31]]},"reference":[{"key":"44579_CR1","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1093\/infdis\/164.1.94","volume":"164","author":"SJ Klebanoff","year":"1991","unstructured":"Klebanoff, S. 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