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In the present study, we explored the protective efficacy of vitamin D<jats:sub>3<\/jats:sub>, retinoic acid and isoprenoid chenodeoxycholic acid (CDCA) combinations against<jats:italic>L<\/jats:italic>.<jats:italic>donovani<\/jats:italic>infected BALB\/c mice. We also probed the immune modulatory response (Th1 &amp; Th2 cytokines) and infection dynamics following experimental infections with drug treated animals. Our results indicate that Vit.D<jats:sub>3<\/jats:sub>\/RA and CDCA\/RA combination treatment led to significant inhibition of parasite load on days 21 and 28 post treatment. Furthermore, there was a marked inhibition of Th2 type immune responses in IL-4, IL-5 and polarization of Th1 biased immunity along with upregulation of IL-1, IFN-\u03b3, and TNF-\u03b1 levels on day 28 post treatment. In addition, mice treated with Vit.D<jats:sub>3<\/jats:sub>\/RA and CDCA\/RA demonstrates here that splenic histological recovery against the virulent challenge of<jats:italic>L<\/jats:italic>.<jats:italic>donovani<\/jats:italic>by day 28 was comparable to control group. The conclusions derived from this study suggests that a combination of vitamin A, D<jats:sub>3<\/jats:sub>and isoprenoids may have a potential immunomodulatory therapeutic role against leishmaniasis.<\/jats:p>","DOI":"10.1038\/s41598-019-44630-4","type":"journal-article","created":{"date-parts":[[2019,6,12]],"date-time":"2019-06-12T10:03:11Z","timestamp":1560333791000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Vitamins (A&amp;D) and Isoprenoid (Chenodeoxycholic acid) molecules are accompanied by Th1 immunostimulatory response and therapeutic cure in vivo: possible antileishmanial drugs"],"prefix":"10.1038","volume":"9","author":[{"given":"Venkateswara Reddy","family":"Gogulamudi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohan Lal","family":"Dubey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deepak","family":"Kaul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donfack Jean","family":"Hubert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramesh","family":"Kandimalla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rakesh","family":"Sehgal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,6,12]]},"reference":[{"key":"44630_CR1","unstructured":"World Health Organization. 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