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The Rusvikunins and their complexes demonstrated concentration-dependent deaggregation and aggregation of washed platelets independent of von Willebrand factor and\/or fibrinogen requirement. At lower concentrations they abolished collagen and ADP-induced platelet aggregation, but at higher concentrations, they progressively decreased the inhibition of ADP-induced aggregation and potentiated the effect of collagen on PRP. Rusvikunin complex\/Rusvikunin-II bound to and induced RGD-independent aggregation of \u03b1-chymotrypsin-treated platelets. Molecular docking studies suggested interaction of Rusvikunin-II and custom peptides with platelet GPIIb\/IIIa receptor, which was validated by spectrofluorometry analysis and ELISA. This study reports, for the first time, an RGD-independent binding of a snake venom component to the platelet GPIIb\/IIIa receptor.<\/jats:p>","DOI":"10.1038\/s41598-019-44767-2","type":"journal-article","created":{"date-parts":[[2019,6,5]],"date-time":"2019-06-05T10:03:07Z","timestamp":1559728987000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["RGD-independent binding of Russell\u2019s Viper venom Kunitz-type protease inhibitors to platelet GPIIb\/IIIa receptor"],"prefix":"10.1038","volume":"9","author":[{"given":"Bhargab","family":"Kalita","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumita","family":"Dutta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashis K.","family":"Mukherjee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,6,5]]},"reference":[{"key":"44767_CR1","unstructured":"WHO. 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