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In this work, we implemented a 3D model of intestine and liver slices from hamsters that were infected <jats:italic>ex vivo<\/jats:italic> with virulent <jats:italic>E<\/jats:italic>. <jats:italic>histolytica<\/jats:italic> trophozoites. Results show preserved histology in both uninfected tissues as well as ulcerations, destruction of the epithelial cells, and inflammatory reaction in intestine slices and formation of micro abscesses, and the presence of amoebae in the sinusoidal spaces and in the interior of central veins in liver slices. The three chemically synthetized compounds T-001, T-011, and T-016, which act as amoebicides <jats:italic>in vitro<\/jats:italic>, were active in both infected tissues, as they decreased the number of trophozoites, and provoked death by disintegration of the amoeba, similar to metronidazole. However, compound T-011 induced signs of cytotoxicity to liver slices. Our results suggest that <jats:italic>ex vivo<\/jats:italic> cultures of precision-cut intestinal and liver slices represent a reliable 3D approach to evaluate novel amoebicidal compounds, and to simultaneously detect their toxicity, while reducing the number of experimental animals commonly required by other model systems.<\/jats:p>","DOI":"10.1038\/s41598-019-44899-5","type":"journal-article","created":{"date-parts":[[2019,6,10]],"date-time":"2019-06-10T10:03:00Z","timestamp":1560160980000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Development of a Novel Ex-vivo 3D Model to Screen Amoebicidal Activity on Infected Tissue"],"prefix":"10.1038","volume":"9","author":[{"given":"Nancy Elena","family":"Guzm\u00e1n-Delgado","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irma Edith","family":"Carranza-Torres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Garc\u00eda-Davis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gildardo","family":"Rivera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Mor\u00e1n-Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia Denys","family":"Betancourt-Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. 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