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Using an unbiased proteomics screen in lung tissue, we identified the membrane protein caveolin -1 (Cav1) as a direct interaction partner of the GR. In <jats:italic>Cav1<\/jats:italic> knockout mice GR transactivates anti-inflammatory genes, including <jats:italic>Dusp1<\/jats:italic>, more than in controls. We therefore determined the role of Cav1 in modulating Gc action in two models of pulmonary inflammation. We first tested innate responses in lung. Loss of <jats:italic>Cav1<\/jats:italic> impaired the inflammatory response to nebulized LPS, increasing cytokine\/chemokine secretion from lung, but impairing neutrophil infiltration. Despite these changes to the inflammatory response, there was no <jats:italic>Cav1<\/jats:italic> effect on anti-inflammatory\u00a0capacity of Gcs. We also tested GR\/Cav1 crosstalk in a model of allergic airway inflammation. <jats:italic>Cav1<\/jats:italic> had a very mild effect on the inflammatory response, but no effect on the Gc response \u2013 with comparable immune cell infiltrate (macrophage, eosinophils, neutrophils), pathological score and PAS positive cells observed between both genotypes. Pursuing the Th2 adaptive immune response further we demonstrate that <jats:italic>Cav1<\/jats:italic> knockout mice retained their ability to expel the intestinal nematode parasite <jats:italic>T.muris<\/jats:italic>, which requires adaptive Th2 immune response for elimination. Therefore, Cav1 regulates innate immune responses in the lung, but does not have an effect on Th2-mediated adaptive immunity in lung or gut. Although we demonstrate that Cav1 regulates GR transactivation of anti-inflammatory genes, this does not translate to an effect on suppression of inflammation <jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1038\/s41598-019-44963-0","type":"journal-article","created":{"date-parts":[[2019,6,12]],"date-time":"2019-06-12T10:02:42Z","timestamp":1560333762000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Caveolin1 interacts with the glucocorticoid receptor in the lung but is dispensable for its anti-inflammatory actions in lung inflammation and Trichuris Muris infection"],"prefix":"10.1038","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1576-8499","authenticated-orcid":false,"given":"G.","family":"Caratti","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Poolman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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