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Its ability to produce phase I (oxidative) metabolites of drugs is associated with cytochrome P450 (CYP) activity; however, almost nothing is known about these enzymes in the fungus. In this paper we report the <jats:italic>in silico<\/jats:italic> analysis of the genome sequence of <jats:italic>C<\/jats:italic>. <jats:italic>elegans<\/jats:italic> B9769, which contains 32 genes putatively coding for CYPs. Based on their predicted amino acid sequences these were classified as belonging to CYP509, 5203, 5208, 5313, 5210, 61 and 51 families. Reverse transcription-quantitative PCR revealed that the gene coding for CYP5313D1 was significantly upregulated when <jats:italic>C<\/jats:italic>. <jats:italic>elegans<\/jats:italic> DSM1908 was cultivated in sabouraud dextrose in contrast to its expression in cells grown in Roswell Park Memorial Institute medium. This corresponded to the fungus\u2019 xenobiotic biotransformation ability when grown in the two media. Heterologous expression of <jats:italic>cyp5313D1<\/jats:italic> in <jats:italic>Pichia pastoris<\/jats:italic> resulted in a recombinant strain that biotransformed flurbiprofen to 4\u2032-hydroxyflurbiprofen, the same metabolite generated by <jats:italic>C<\/jats:italic>. <jats:italic>elegans<\/jats:italic> cultures. This is the first report of a xenobiotic-biotransforming CYP from this biotechnologically important fungus.<\/jats:p>","DOI":"10.1038\/s41598-019-45706-x","type":"journal-article","created":{"date-parts":[[2019,6,25]],"date-time":"2019-06-25T21:52:50Z","timestamp":1561499570000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["The CYPome of the model xenobiotic-biotransforming fungus Cunninghamella elegans"],"prefix":"10.1038","volume":"9","author":[{"given":"William","family":"Palmer-Brown","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9294-6578","authenticated-orcid":false,"given":"Ra\u00fal","family":"Miranda-CasoLuengo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4992-4979","authenticated-orcid":false,"given":"Kenneth H.","family":"Wolfe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin P.","family":"Byrne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2137-3338","authenticated-orcid":false,"given":"Cormac D.","family":"Murphy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,6,25]]},"reference":[{"key":"45706_CR1","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1007\/s10529-014-1653-8","volume":"37","author":"CD Murphy","year":"2015","unstructured":"Murphy, C. 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