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Approximately 15\u201325 million people are chronically infected with HDV. As a satellite virus of the human hepatitis B virus (HBV), HDV uses the HBV-encoded envelope proteins for egress from and <jats:italic>de novo<\/jats:italic> entry into hepatocytes. So far, <jats:italic>in vitro<\/jats:italic> production of HDV particles is restricted to co-transfection of cells with HDV\/HBV encoding cDNAs. This approach has several limitations. In this study, we established HuH7-END cells, which continuously secrete infectious HDV virions. The cell line was generated through stepwise stable integration of the cDNA of the HDV antigenome, the genes for the HBV envelope proteins and the HBV\/HDV receptor NTCP. We found that HuH7-END cells release infectious HDV particles up to 400 million copies\/milliliter and support virus spread to co-cultured cells. Due to the expression of NTCP, HuH7-END cells are also susceptible to <jats:italic>de novo<\/jats:italic> HDV entry. Virus production is stable for &gt;16 passages and can be scaled up for preparation of large HDV virus stocks. Finally, HuH7-END cells are suitable for screening of antiviral drugs targeting HDV replication. In summary, the HuH7-END cell line provides a novel tool to study HDV replication <jats:italic>in vitro<\/jats:italic>.<\/jats:p>","DOI":"10.1038\/s41598-019-46493-1","type":"journal-article","created":{"date-parts":[[2019,7,10]],"date-time":"2019-07-10T10:03:11Z","timestamp":1562752991000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Generation and characterization of a stable cell line persistently replicating and secreting the human hepatitis delta virus"],"prefix":"10.1038","volume":"9","author":[{"given":"Yi","family":"Ni","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenfeng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Engelskircher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg","family":"Verch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0482-4387","authenticated-orcid":false,"given":"Thomas","family":"Tu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian A.","family":"Lempp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Urban","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,7,10]]},"reference":[{"key":"46493_CR1","doi-asserted-by":"publisher","first-page":"997","DOI":"10.1136\/gut.18.12.997","volume":"18","author":"M Rizzetto","year":"1977","unstructured":"Rizzetto, M. et al. 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The other authors in this study declare nothing to disclose.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"10021"}}