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Clinical symptoms are non-distinct and diagnosis mainly relies on the detection of microfilariae in skin or blood. Species-specific DNA repeat sequences have been used as highly sensitive biomarkers for filarial nematodes. We have developed a bioinformatic pipeline to mine Illumina reads obtained from sequencing\n                    <jats:italic>M<\/jats:italic>\n                    .\n                    <jats:italic>perstans<\/jats:italic>\n                    and\n                    <jats:italic>M<\/jats:italic>\n                    .\n                    <jats:italic>ozzardi<\/jats:italic>\n                    genomic DNA for new repeat biomarker candidates which were used to develop loop-mediated isothermal amplification (LAMP) diagnostic tests. The\n                    <jats:italic>M<\/jats:italic>\n                    .\n                    <jats:italic>perstans<\/jats:italic>\n                    assay based on the Mp419 repeat has a limit of detection of 0.1\u2009pg, equivalent of 1\/1000\n                    <jats:sup>th<\/jats:sup>\n                    of a microfilaria, while the\n                    <jats:italic>M<\/jats:italic>\n                    .\n                    <jats:italic>ozzardi<\/jats:italic>\n                    assay based on the Mo2 repeat can detect as little as 0.01\u2009pg. Both LAMP tests possess remarkable species-specificity as they did not amplify non-target DNAs from closely related filarial species, human or vectors. We show that both assays perform successfully on infected human samples. Additionally, we demonstrate the suitability of Mp419 to detect\n                    <jats:italic>M<\/jats:italic>\n                    .\n                    <jats:italic>perstans<\/jats:italic>\n                    infection in\n                    <jats:italic>Culicoides<\/jats:italic>\n                    midges. These new tools are field deployable and suitable for the surveillance of these understudied filarial infections.\n                  <\/jats:p>","DOI":"10.1038\/s41598-019-46550-9","type":"journal-article","created":{"date-parts":[[2019,7,16]],"date-time":"2019-07-16T06:03:23Z","timestamp":1563257003000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["In Silico Identification of Novel Biomarkers and Development of New Rapid Diagnostic Tests for the Filarial Parasites Mansonella perstans and Mansonella ozzardi"],"prefix":"10.1038","volume":"9","author":[{"given":"C. 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