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This study characterized a transferable multidrug resistance plasmid harboring\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>IMP-26<\/jats:sub>\n                    from one\n                    <jats:italic>Enterobacter cloacae<\/jats:italic>\n                    bloodstream isolate in Shanghai and investigated the genetic environment of resistance genes. The isolate was subjected to antimicrobial susceptibility testing and multilocus sequence typing using broth microdilution method, Etest and PCR. The plasmid was analyzed through conjugation experiments, S1-nuclease pulsed-field gel electrophoresis and hybridization. Whole genome sequencing and sequence analysis was conducted for further investigation of the plasmid.\n                    <jats:italic>E. cloacae<\/jats:italic>\n                    RJ702, belonging to ST528 and carrying\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>IMP-26<\/jats:sub>\n                    ,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>DHA-1<\/jats:sub>\n                    ,\n                    <jats:italic>qnrB<\/jats:italic>\n                    4 and\n                    <jats:italic>fosA5<\/jats:italic>\n                    , was resistant to almost all \u03b2-lactams, but susceptible to quinolones and tigecycline. The transconjugant inherited the multidrug resistance. The resistance genes were located on a 329,420-bp IncHI2 conjugative plasmid pIMP26 (ST1 subtype), which contained\n                    <jats:italic>trhK\/trhV<\/jats:italic>\n                    ,\n                    <jats:italic>tra<\/jats:italic>\n                    ,\n                    <jats:italic>parA<\/jats:italic>\n                    and\n                    <jats:italic>stbA<\/jats:italic>\n                    family operon. The\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>IMP-26<\/jats:sub>\n                    was arranged following\n                    <jats:italic>intI1<\/jats:italic>\n                    . The\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>DHA-1<\/jats:sub>\n                    and\n                    <jats:italic>qnrB4<\/jats:italic>\n                    cluster was the downstream of IS\n                    <jats:italic>CR1<\/jats:italic>\n                    , same as that in p505108-MDR. The\n                    <jats:italic>fosA5<\/jats:italic>\n                    cassette was mediated by IS\n                    <jats:italic>4<\/jats:italic>\n                    . This was the first report on complete nucleotide of a\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>IMP-26<\/jats:sub>\n                    -carrying plasmid in\n                    <jats:italic>E. cloacae<\/jats:italic>\n                    in China. Plasmid pIMP26 hosted high phylogenetic mosaicism, transferability and plasticity.\n                  <\/jats:p>","DOI":"10.1038\/s41598-019-46777-6","type":"journal-article","created":{"date-parts":[[2019,7,15]],"date-time":"2019-07-15T06:50:58Z","timestamp":1563173458000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["A Multidrug Resistance Plasmid pIMP26, Carrying blaIMP-26, fosA5, blaDHA-1, and qnrB4 in Enterobacter cloacae"],"prefix":"10.1038","volume":"9","author":[{"given":"Su","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaixin","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuzhen","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianyan","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feifei","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxing","family":"Ni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyong","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizhong","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,7,15]]},"reference":[{"key":"46777_CR1","doi-asserted-by":"publisher","first-page":"609","DOI":"10.1093\/jac\/dkn534","volume":"63","author":"K Kaier","year":"2009","unstructured":"Kaier, K., Frank, U., Hagist, C., Conrad, A. & Meyer, E. 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