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Preoperative serum samples of a training (TC) (n\u2009=\u2009219) and a validation cohort (VC) (n\u2009=\u2009168) were analyzed via ELISA to determine PlGF, EGF, VEGF, Ang1, PDGF-A, PDGF-B, IL-8 and bFGF levels. In addition, survival was correlated with PlGF and EGF expression measured by microarray and RNAseq in two publicly available, independent cohorts (n\u2009=\u2009550 and n\u2009=\u2009463, respectively). Prognostic values for overall (OS) and disease-free survival (DFS) were determined using uni- and multivariate Cox proportional hazard analyses. Elevated PlGF is predictive for impaired OS (TC: HR 1.056; <jats:italic>p<\/jats:italic>\u2009=\u20090.046; VC: HR 1.093; <jats:italic>p<\/jats:italic>\u2009=\u20090.001) and DFS (TC: HR 1.052; <jats:italic>p<\/jats:italic>\u2009=\u20090.029; VC: HR 1.091; <jats:italic>p<\/jats:italic>\u2009=\u20090.009). Conversely, elevated EGF is associated with favorable DFS (TC: HR 0.998; <jats:italic>p<\/jats:italic>\u2009=\u20090.045; VC: HR 0.998; <jats:italic>p<\/jats:italic>\u2009=\u20090.018) but not OS (TC: <jats:italic>p<\/jats:italic>\u2009=\u20090.201; VC: <jats:italic>p<\/jats:italic>\u2009=\u20090.453). None of the other angiogenic cytokines correlated with prognosis. The prognostic value of PlGF (OS\u2009+\u2009DFS) and EGF (DFS) was confirmed in both independent retrospective cohorts. Serum PlGF and EGF may serve as prognostic markers in non-metastatic CRC.<\/jats:p>","DOI":"10.1038\/s41598-019-47429-5","type":"journal-article","created":{"date-parts":[[2019,7,29]],"date-time":"2019-07-29T10:21:36Z","timestamp":1564395696000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Serum PlGF and EGF are independent prognostic markers in non-metastatic colorectal cancer"],"prefix":"10.1038","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0012-3177","authenticated-orcid":false,"given":"Sebastian","family":"Sch\u00f6lch","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9226-5337","authenticated-orcid":false,"given":"Andreas","family":"Bogner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Bork","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1133-2134","authenticated-orcid":false,"given":"Mohammad","family":"Rahbari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bal\u00e1zs","family":"Gy\u0151rffy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Schneider","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Reissfelder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"Weitz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nuh N.","family":"Rahbari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,7,29]]},"reference":[{"key":"47429_CR1","doi-asserted-by":"publisher","first-page":"87","DOI":"10.3322\/caac.21262","volume":"65","author":"LA Torre","year":"2015","unstructured":"Torre, L. 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