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However, previous <jats:italic>in vivo<\/jats:italic> measurements of MCT velocity using this method were lower than those from alternate methods. We hypothesise this was due to the surface chemistry of the uncoated particles. We investigated the effect of particle surface coating on MCT marker performance by measuring the velocity of uncoated, positively-charged (aminated; NH<jats:sub>2<\/jats:sub>), and negatively-charged (carboxylated; COOH) particles. The effect of aerosolised hypertonic saline (HS) was also investigated, as previous <jats:italic>in vivo<\/jats:italic> measurements showed HS significantly increased MCT rate. PCXI experiments were performed using an <jats:italic>ex vivo<\/jats:italic> rat tracheal imaging setup. Prior to aerosol delivery there was little movement of the uncoated particles, whilst the NH<jats:sub>2<\/jats:sub> and COOH particles moved with MCT rates similar to those previously reported. After application of HS the uncoated and COOH particle velocity increased and NH<jats:sub>2<\/jats:sub> decreased. This experiment validated the use of COOH particles as MCT marker particles over the uncoated and NH<jats:sub>2<\/jats:sub> coated particles. Our results suggest that future experiments measuring MCT using synchrotron PCXI should use COOH coated marker particles for more accurate MCT quantification.<\/jats:p>","DOI":"10.1038\/s41598-019-47465-1","type":"journal-article","created":{"date-parts":[[2019,7,29]],"date-time":"2019-07-29T10:21:36Z","timestamp":1564395696000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Particle coating alters mucociliary transit in excised rat trachea: A synchrotron X-ray imaging study"],"prefix":"10.1038","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4340-2547","authenticated-orcid":false,"given":"Mark","family":"Gardner","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"McCarron","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaye","family":"Morgan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Parsons","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Donnelley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,7,29]]},"reference":[{"key":"47465_CR1","doi-asserted-by":"publisher","first-page":"571","DOI":"10.1172\/JCI0215217","volume":"109","author":"MR Knowles","year":"2002","unstructured":"Knowles, M. 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