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In the present study, a retroviral solution (1.5\u20132.0\u2009\u00d7\u200910<jats:sup>7<\/jats:sup> \/ul) of mock pMX-GFP (n\u2009=\u200913) or pMX-Ascl1\/Sox2\/NeuroD1 (ASN) (n\u2009=\u200914) was directly injected into the ipsilateral striatum and cortex 3 days after 30\u2009min of transient cerebral ischemia. The reprogrammed cells first expressed neuronal progenitor marker Dcx 7 and 21 days after viral injection, then expressed mature neuronal marker NeuN. This was accompanied by morphological changes, including long processes and synapse-like structures, 49 days after viral injection. Meanwhile, therapeutic improvement was not detected both in clinical scores or infarct volume. The present study provides a future novel self-repair strategy for ischemic stroke with beneficial modifications of the inducer-suppressor balance.<\/jats:p>","DOI":"10.1038\/s41598-019-47482-0","type":"journal-article","created":{"date-parts":[[2019,7,29]],"date-time":"2019-07-29T10:21:36Z","timestamp":1564395696000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["In vivo direct reprogramming of glial linage to mature neurons after cerebral ischemia"],"prefix":"10.1038","volume":"9","author":[{"given":"Toru","family":"Yamashita","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingwei","family":"Shang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumiko","family":"Nakano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryuta","family":"Morihara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kota","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mami","family":"Takemoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nozomi","family":"Hishikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuyuki","family":"Ohta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Abe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,7,29]]},"reference":[{"key":"47482_CR1","doi-asserted-by":"publisher","first-page":"480","DOI":"10.1161\/01.STR.19.4.480","volume":"19","author":"K Abe","year":"1988","unstructured":"Abe, K., Yuki, S. & Kogure, K. 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