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Therefore, major efforts have been made in the last decade to unravel key molecular signals that can drive salivary gland (SG) regeneration and functional restoration. However, the earliest molecular determinants that accompany SG regeneration remain incompletely defined. The present study examined the initial mitogenic events marking the regenerative response of the murine submandibular gland (SMG), following innate immune-mediated injury. Local intraductal administration of the synthetic double stranded (ds) RNA polyinosinic-polycytidylic acid (poly (I:C)) widely, but transiently, depleted the acinar and progenitor cells, 24\u2009hours post poly (I:C) introduction. While the progenitor and duct cells started to proliferate and expand at 72\u2009hours, the Mist1-positve acinar cells did not re-appear until 96\u2009hours post poly (I:C) injury. The cellular replenishment during regeneration involved significant upregulation of the cell cycle promoter Aurora kinase B (AURKB). AURKB, which is expressed in healthy proliferating and cancerous cells, is a serine\/threonine protein kinase, well known to orchestrate key events in cell division and cytokinesis. However, the expression and role of AURKB in regeneration of post mitotic salivary gland cells has not been previously explored. <jats:italic>In vivo<\/jats:italic> inhibition of AURKB using the selective inhibitor Barasertib (AZD1152-HQPA) interfered with SMG recovery from the transient, but severe poly (I:C)-mediated injury and cellular depletion. AURKB deficiency during regeneration of the injured tissues: disrupted cell cycle progression, repressed renewal of Mist1-positive acinar cells and prevented recovery of salivary secretion. The knowledge gained in this study may be utilized in the development of therapeutic targets for irreversible salivary gland disease.<\/jats:p>","DOI":"10.1038\/s41598-019-47762-9","type":"journal-article","created":{"date-parts":[[2019,8,5]],"date-time":"2019-08-05T10:02:59Z","timestamp":1564999379000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Salivary glands require Aurora Kinase B\u00a0for regeneration after transient innate immune-mediated injury"],"prefix":"10.1038","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9025-3885","authenticated-orcid":false,"given":"Abeer","family":"Shaalan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5684-841X","authenticated-orcid":false,"given":"Gordon","family":"Proctor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,8,5]]},"reference":[{"key":"47762_CR1","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1111\/jop.12663","volume":"47","author":"A Shaalan","year":"2018","unstructured":"Shaalan, A., Carpenter, G. & Proctor, G. 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