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The serine protease HtrA, an important secreted virulence factor, disrupts the gastric epithelium, which enables\n                    <jats:italic>H<\/jats:italic>\n                    .\n                    <jats:italic>pylori<\/jats:italic>\n                    to transmigrate across the epithelium and inject the oncogenic CagA protein into host cells. The function of periplasmic HtrA for the\n                    <jats:italic>H<\/jats:italic>\n                    .\n                    <jats:italic>pylori<\/jats:italic>\n                    cell is unknown, mainly due to unavailability of the\n                    <jats:italic>htrA<\/jats:italic>\n                    mutants. In fact,\n                    <jats:italic>htrA<\/jats:italic>\n                    has been described as an essential gene in this bacterium. We have screened 100 worldwide\n                    <jats:italic>H<\/jats:italic>\n                    .\n                    <jats:italic>pylori<\/jats:italic>\n                    isolates and show that only in the N6 strain it was possible to delete\n                    <jats:italic>htrA<\/jats:italic>\n                    or mutate the\n                    <jats:italic>htrA<\/jats:italic>\n                    gene to produce proteolytically inactive HtrA. We have sequenced the wild-type and mutant chromosomes and we found that inactivation of\n                    <jats:italic>htrA<\/jats:italic>\n                    is associated with mutations in SecA \u2013 a component of the Sec translocon apparatus used to translocate proteins from the cytoplasm into the periplasm. The cooperation of SecA and HtrA has been already suggested in\n                    <jats:italic>Streptococcus pneumonia<\/jats:italic>\n                    , in which these two proteins co-localize. Hence, our results pinpointing a potential functional relationship between HtrA and the Sec translocon in\n                    <jats:italic>H<\/jats:italic>\n                    .\n                    <jats:italic>pylori<\/jats:italic>\n                    possibly indicate for the more general mechanism responsible to maintain bacterial periplasmic homeostasis.\n                  <\/jats:p>","DOI":"10.1038\/s41598-019-48030-6","type":"journal-article","created":{"date-parts":[[2019,8,13]],"date-time":"2019-08-13T06:02:28Z","timestamp":1565676148000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Establishment of serine protease htrA mutants in Helicobacter pylori is associated with secA mutations"],"prefix":"10.1038","volume":"9","author":[{"given":"Anna","family":"Zawilak-Pawlik","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Urszula","family":"Zarzecka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorota","family":"\u017by\u0142a-Uklejewicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakub","family":"Lach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9752-4270","authenticated-orcid":false,"given":"Dominik","family":"Strapagiel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Tegtmeyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manja","family":"B\u00f6hm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Backert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Skorko-Glonek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,8,13]]},"reference":[{"key":"48030_CR1","doi-asserted-by":"publisher","first-page":"977","DOI":"10.2174\/1381612811319060003","volume":"19","author":"J Skorko-Glonek","year":"2013","unstructured":"Skorko-Glonek, J. et al. 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