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The host plasma membrane serves as the portal of entry for <jats:italic>Leishmania<\/jats:italic> to gain access to the cellular interior. Although several host cell membrane receptors have been shown to be involved in the entry of <jats:italic>Leishmania donovani<\/jats:italic> into host cells, the endocytic pathway involved in the internalization of the parasite is not known. In this work, we explored the endocytic pathway involved in the entry of <jats:italic>Leishmania donovani<\/jats:italic> into host macrophages, utilizing specific inhibitors against two major pathways of internalization, <jats:italic>i.e<\/jats:italic>., clathrin- and caveolin-mediated endocytosis. We show that pitstop 2, an inhibitor for clathrin-mediated endocytosis, does not affect the entry of <jats:italic>Leishmania donovani<\/jats:italic> promastigotes into host macrophages. Interestingly, a significant reduction in internalization was observed upon treatment with genistein, an inhibitor for caveolin-mediated endocytosis. These results are supported by a similar trend in intracellular amastigote load within host macrophages. These results suggest that <jats:italic>Leishmania donovani<\/jats:italic> utilizes caveolin-mediated endocytosis to internalize into host cells. Our results provide novel insight into the mechanism of phagocytosis of <jats:italic>Leishmania donovani<\/jats:italic> into host cells and assume relevance in the development of novel therapeutics against leishmanial infection.<\/jats:p>","DOI":"10.1038\/s41598-019-49007-1","type":"journal-article","created":{"date-parts":[[2019,9,2]],"date-time":"2019-09-02T18:11:06Z","timestamp":1567447866000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Leishmania donovani Internalizes into Host Cells via Caveolin-mediated Endocytosis"],"prefix":"10.1038","volume":"9","author":[{"given":"G. 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