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The actinobacterial strain PM33 yields maximum amount of antifouling compounds through agar surface fermentation. In optimization, carbohydrates such as glucose, fructose and xylose, are suitable for maximum production of the active compound. In addition, other compounds such as malt extract, glutamine, and sodium chloride concentrations (2.5, 5 and 7.5%) and parameters such as pH 7.0 and temperature range 30\u2009\u00b0C to 40\u2009\u00b0C enhanced the production of antifouling metabolite. The antifouling metabolite was extracted in ethyl acetate. TLC and bioautography was used to separate and detect the antifouling metabolite present in the crude extract. The physico chemical features revealed that the antifouling metabolite PM33 \u2013 B as taxifolin (C<jats:sub>15<\/jats:sub>H<jats:sub>12<\/jats:sub>O<jats:sub>7<\/jats:sub>). The purified taxifolin was found to be active against biofouling bacteria, algal spore germination and mollusc foot adherence, respectively. Toxicity nature of taxifolin was also determined by adopting zebrafish embryos. The taxifolin isolated from mangrove-derived <jats:italic>Streptomyces sampsonii<\/jats:italic> PM33 is a promising candidate for the development of eco-friendly antifouling preparation.<\/jats:p>","DOI":"10.1038\/s41598-019-49478-2","type":"journal-article","created":{"date-parts":[[2019,9,10]],"date-time":"2019-09-10T10:04:12Z","timestamp":1568109852000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Isolation, characterization and identification of antibiofouling metabolite from mangrove derived Streptomyces sampsonii PM33"],"prefix":"10.1038","volume":"9","author":[{"given":"Venugopal","family":"Gopikrishnan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manikkam","family":"Radhakrishnan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thangavel","family":"Shanmugasundaram","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meganathan P.","family":"Ramakodi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramasamy","family":"Balagurunathan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,9,10]]},"reference":[{"key":"49478_CR1","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1038\/ncomms1251","volume":"2","author":"JA Callow","year":"2011","unstructured":"Callow, J. 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