{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T15:12:58Z","timestamp":1788189178722,"version":"build-2803163510"},"reference-count":43,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2019,9,10]],"date-time":"2019-09-10T00:00:00Z","timestamp":1568073600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2019,9,10]],"date-time":"2019-09-10T00:00:00Z","timestamp":1568073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SFB118 (A04 &amp; S01)"],"award-info":[{"award-number":["SFB118 (A04 &amp; S01)"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["GRK 1871\/8"],"award-info":[{"award-number":["GRK 1871\/8"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Diabetes-induced hyperglycemia has previously been shown to impact on male sub-\/infertility, however, still little is known about the underlying mechanisms. In the present study we have addressed three major biochemical pathways implicated in the pathogenesis of hyperglycemia induced organ damage (the advanced glycation end product (AGE) formation pathway, the diacylglycerol-protein kinase C pathway (PKC), and the polyol pathway) in both testis and epididymis of the\n                    <jats:italic>Ins2<\/jats:italic>\n                    <jats:sup>Akita<\/jats:sup>\n                    mouse model of Type 1 diabetes (T1DM). Hyperglycemia activated both the PKC and the polyol pathway in a significant and progressive manner within the testis, but not within the epididymis. While the AGE receptor was ubiquitiously expressed in the testis, concentrations of precursor methylglyoxal and AGE carboxymethyllysine were increased in both epididymis and testis in diabetic mice. However, AGEs did not activate intracellular pathways of ERK1, ERK2, Rela, Nrf-2, IkBkB, NFkB except CDC42, Akt1. In conclusion, two of the major pathways of hyperglycemia-induced organ damage were clearly activated within the testis of T1DM mice. This provides therapeutical opportunities in the treatment of diabetic male reproductive dysfunction.\n                  <\/jats:p>","DOI":"10.1038\/s41598-019-49600-4","type":"journal-article","created":{"date-parts":[[2019,9,10]],"date-time":"2019-09-10T06:03:36Z","timestamp":1568095416000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["Hyperglycemia induces spermatogenic disruption via major pathways of diabetes pathogenesis"],"prefix":"10.1038","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4685-535X","authenticated-orcid":false,"given":"Constanze C.","family":"Maresch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dina C.","family":"Stute","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Fleming","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jihong","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Peter","family":"Hammes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0870-8164","authenticated-orcid":false,"given":"Thomas","family":"Linn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,9,10]]},"reference":[{"key":"49600_CR1","doi-asserted-by":"publisher","first-page":"1871","DOI":"10.1093\/humrep\/dem077","volume":"22","author":"IM Agbaje","year":"2007","unstructured":"Agbaje, I. M. et al. Insulin dependant diabetes mellitus: implications for male reproductive function. Human reproduction (Oxford, England) 22, 1871\u20131877, https:\/\/doi.org\/10.1093\/humrep\/dem077 (2007).","journal-title":"Human reproduction (Oxford, England)"},{"key":"49600_CR2","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1016\/j.diabres.2013.11.002","volume":"103","author":"L Guariguata","year":"2014","unstructured":"Guariguata, L. et al. Global estimates of diabetes prevalence for 2013 and projections for 2035. Diabetes research and clinical practice 103, 137\u2013149, https:\/\/doi.org\/10.1016\/j.diabres.2013.11.002 (2014).","journal-title":"Diabetes research and clinical practice"},{"key":"49600_CR3","doi-asserted-by":"crossref","unstructured":"Silink, M. Childhood diabetes: a global perspective. Horm Res 57 Suppl 1, 1\u20135, doi:53304 (2002).","DOI":"10.1159\/000053304"},{"key":"49600_CR4","doi-asserted-by":"publisher","first-page":"1047","DOI":"10.2337\/diacare.27.5.1047","volume":"27","author":"S Wild","year":"2004","unstructured":"Wild, S., Roglic, G., Green, A., Sicree, R. & King, H. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care 27, 1047\u20131053 (2004).","journal-title":"Diabetes Care"},{"key":"49600_CR5","unstructured":"World Health Organization. Global report on diabetes (2016)."},{"key":"49600_CR6","doi-asserted-by":"publisher","first-page":"813","DOI":"10.1038\/414813a","volume":"414","author":"M Brownlee","year":"2001","unstructured":"Brownlee, M. Biochemistry and molecular cell biology of diabetic complications. Nature 414, 813\u2013820, https:\/\/doi.org\/10.1038\/414813a (2001).","journal-title":"Nature"},{"key":"49600_CR7","doi-asserted-by":"publisher","first-page":"12222","DOI":"10.1073\/pnas.97.22.12222","volume":"97","author":"XL Du","year":"2000","unstructured":"Du, X. L. et al. Hyperglycemia-induced mitochondrial superoxide overproduction activates the hexosamine pathway and induces plasminogen activator inhibitor-1 expression by increasing Sp1 glycosylation. Proceedings of the National Academy of Sciences of the United States of America 97, 12222\u201312226, https:\/\/doi.org\/10.1073\/pnas.97.22.12222 (2000).","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"49600_CR8","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1038\/35008121","volume":"404","author":"T Nishikawa","year":"2000","unstructured":"Nishikawa, T. et al. Normalizing mitochondrial superoxide production blocks three pathways of hyperglycaemic damage. Nature 404, 787\u2013790, https:\/\/doi.org\/10.1038\/35008121 (2000).","journal-title":"Nature"},{"key":"49600_CR9","doi-asserted-by":"publisher","first-page":"e23992","DOI":"10.4161\/tisb.23992","volume":"1","author":"MG Alves","year":"2013","unstructured":"Alves, M. G., Martins, A. D., Cavaco, J. E., Socorro, S. & Oliveira, P. F. Diabetes, insulin-mediated glucose metabolism and Sertoli\/blood-testis barrier function. Tissue Barriers 1, e23992, https:\/\/doi.org\/10.4161\/tisb.23992 (2013).","journal-title":"Tissue Barriers"},{"key":"49600_CR10","doi-asserted-by":"publisher","first-page":"626","DOI":"10.1016\/j.bbadis.2013.01.011","volume":"1832","author":"MG Alves","year":"2013","unstructured":"Alves, M. G. et al. Molecular mechanisms beyond glucose transport in diabetes-related male infertility. Biochimica et biophysica acta 1832, 626\u2013635, https:\/\/doi.org\/10.1016\/j.bbadis.2013.01.011 (2013).","journal-title":"Biochimica et biophysica acta"},{"key":"49600_CR11","doi-asserted-by":"publisher","first-page":"706","DOI":"10.1002\/j.1939-4640.2004.tb02845.x","volume":"25","author":"J Ballester","year":"2004","unstructured":"Ballester, J. et al. Insulin-dependent diabetes affects testicular function by FSH- and LH-linked mechanisms. J Androl 25, 706\u2013719 (2004).","journal-title":"J Androl"},{"key":"49600_CR12","doi-asserted-by":"publisher","first-page":"147","DOI":"10.2174\/1573399810666140606111745","volume":"10","author":"RN Jangir","year":"2014","unstructured":"Jangir, R. N. & Jain, G. C. Diabetes mellitus induced impairment of male reproductive functions: a review. Curr Diabetes Rev 10, 147\u2013157 (2014).","journal-title":"Curr Diabetes Rev"},{"key":"49600_CR13","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1111\/j.1365-2605.2007.00849.x","volume":"32","author":"C Mallidis","year":"2009","unstructured":"Mallidis, C. et al. Advanced glycation end products accumulate in the reproductive tract of men with diabetes. Int J Androl 32, 295\u2013305, https:\/\/doi.org\/10.1111\/j.1365-2605.2007.00849.x (2009).","journal-title":"Int J Androl"},{"key":"49600_CR14","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1007\/BF02913088","volume":"21","author":"M Maneesh","year":"2006","unstructured":"Maneesh, M., Jayalakshmi, H., Singh, T. A. & Chakrabarti, A. Impaired hypothalamic-pituitary-gonadal axis function in men with diabetes mellitus. Indian J Clin Biochem 21, 165\u2013168, https:\/\/doi.org\/10.1007\/BF02913088 (2006).","journal-title":"Indian J Clin Biochem"},{"key":"49600_CR15","doi-asserted-by":"publisher","first-page":"948","DOI":"10.4103\/1008-682X.150844","volume":"17","author":"GL Ding","year":"2015","unstructured":"Ding, G. L. et al. The effects of diabetes on male fertility and epigenetic regulation during spermatogenesis. Asian J Androl 17, 948\u2013953, https:\/\/doi.org\/10.4103\/1008-682X.150844 (2015).","journal-title":"Asian J Androl"},{"key":"49600_CR16","doi-asserted-by":"publisher","first-page":"2169","DOI":"10.1093\/humrep\/dem156","volume":"22","author":"C Mallidis","year":"2007","unstructured":"Mallidis, C. et al. Distribution of the receptor for advanced glycation end products in the human male reproductive tract: prevalence in men with diabetes mellitus. Human reproduction (Oxford, England) 22, 2169\u20132177, https:\/\/doi.org\/10.1093\/humrep\/dem156 (2007).","journal-title":"Human reproduction (Oxford, England)"},{"key":"49600_CR17","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.diabres.2010.09.024","volume":"91","author":"J Karimi","year":"2011","unstructured":"Karimi, J., Goodarzi, M. T., Tavilani, H., Khodadadi, I. & Amiri, I. Relationship between advanced glycation end products and increased lipid peroxidation in semen of diabetic men. Diabetes research and clinical practice 91, 61\u201366, https:\/\/doi.org\/10.1016\/j.diabres.2010.09.024 (2011).","journal-title":"Diabetes research and clinical practice"},{"issue":"Suppl 1","key":"49600_CR18","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1111\/j.1439-0272.2011.01178.x","volume":"44","author":"J Karimi","year":"2012","unstructured":"Karimi, J., Goodarzi, M. T., Tavilani, H., Khodadadi, I. & Amiri, I. Increased receptor for advanced glycation end products in spermatozoa of diabetic men and its association with sperm nuclear DNA fragmentation. Andrologia 44(Suppl 1), 280\u2013286, https:\/\/doi.org\/10.1111\/j.1439-0272.2011.01178.x (2012).","journal-title":"Andrologia"},{"key":"49600_CR19","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-27108-7","volume":"8","author":"C Nevin","year":"2018","unstructured":"Nevin, C. et al. Investigating the Glycating Effects of Glucose, Glyoxal and Methylglyoxal on Human Sperm. Scientific reports 8, 9002, https:\/\/doi.org\/10.1038\/s41598-018-27108-7 (2018).","journal-title":"Scientific reports"},{"key":"49600_CR20","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/j.mce.2017.02.020","volume":"446","author":"CC Maresch","year":"2017","unstructured":"Maresch, C. C. et al. Hyperglycemia is associated with reduced testicular function and activin dysregulation in the Ins2Akita+\/- mouse model of type 1 diabetes. Mol Cell Endocrinol 446, 91\u2013101, https:\/\/doi.org\/10.1016\/j.mce.2017.02.020 (2017).","journal-title":"Mol Cell Endocrinol"},{"key":"49600_CR21","doi-asserted-by":"publisher","first-page":"10595","DOI":"10.1093\/nar\/gkx705","volume":"45","author":"V Kumar","year":"2017","unstructured":"Kumar, V. et al. Homeostatic nuclear RAGE-ATM interaction is essential for efficient DNA repair. Nucleic Acids Res 45, 10595\u201310613, https:\/\/doi.org\/10.1093\/nar\/gkx705 (2017).","journal-title":"Nucleic Acids Res"},{"key":"49600_CR22","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1093\/humupd\/dmx033","volume":"24","author":"CC Maresch","year":"2018","unstructured":"Maresch, C. C. et al. Diabetes-induced hyperglycemia impairs male reproductive function: a systematic review. Hum Reprod Update 24, 86\u2013105, https:\/\/doi.org\/10.1093\/humupd\/dmx033 (2018).","journal-title":"Hum Reprod Update"},{"key":"49600_CR23","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/j.mce.2017.02.020","volume":"446","author":"CC Maresch","year":"2017","unstructured":"Maresch, C. C. et al. Hyperglycemia is associated with reduced testicular function and activin dysregulation in the Ins2(Akita+\/-) mouse model of type 1 diabetes. Mol Cell Endocrinol 446, 91\u2013101, https:\/\/doi.org\/10.1016\/j.mce.2017.02.020 (2017).","journal-title":"Mol Cell Endocrinol"},{"key":"49600_CR24","doi-asserted-by":"publisher","first-page":"776","DOI":"10.1016\/j.acthis.2016.09.008","volume":"118","author":"M Tripathi","year":"2016","unstructured":"Tripathi, M., Singh, A. P., Gupta, G. & Rajender, S. Concomitant and discrete expressions of aldose reductase and sorbitol dehydrogenase in the male reproductive tract. Acta Histochem 118, 776\u2013783, https:\/\/doi.org\/10.1016\/j.acthis.2016.09.008 (2016).","journal-title":"Acta Histochem"},{"key":"49600_CR25","doi-asserted-by":"publisher","first-page":"448","DOI":"10.1111\/j.1432-1033.1995.tb20408.x","volume":"227","author":"T Gui","year":"1995","unstructured":"Gui, T., Tanimoto, T., Kokai, Y. & Nishimura, C. Presence of a closely related subgroup in the aldo-ketoreductase family of the mouse. European journal of biochemistry 227, 448\u2013453 (1995).","journal-title":"European journal of biochemistry"},{"issue":"Pt 1","key":"49600_CR26","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1042\/bj3180119","volume":"318","author":"A Hoshi","year":"1996","unstructured":"Hoshi, A. et al. Glycation and inactivation of sorbitol dehydrogenase in normal and diabetic rats. The Biochemical journal 318(Pt 1), 119\u2013123 (1996).","journal-title":"The Biochemical journal"},{"key":"49600_CR27","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1095\/biolreprod.108.068882","volume":"80","author":"W Cao","year":"2009","unstructured":"Cao, W., Aghajanian, H. K., Haig-Ladewig, L. A. & Gerton, G. L. Sorbitol can fuel mouse sperm motility and protein tyrosine phosphorylation via sorbitol dehydrogenase. Biol Reprod 80, 124\u2013133, https:\/\/doi.org\/10.1095\/biolreprod.108.068882 (2009).","journal-title":"Biol Reprod"},{"key":"49600_CR28","doi-asserted-by":"publisher","first-page":"1319","DOI":"10.1161\/CIRCRESAHA.110.217117","volume":"106","author":"P Geraldes","year":"2010","unstructured":"Geraldes, P. & King, G. L. Activation of protein kinase C isoforms and its impact on diabetic complications. Circ Res 106, 1319\u20131331, https:\/\/doi.org\/10.1161\/CIRCRESAHA.110.217117 (2010).","journal-title":"Circ Res"},{"key":"49600_CR29","doi-asserted-by":"publisher","first-page":"859","DOI":"10.2337\/diabetes.47.6.859","volume":"47","author":"D Koya","year":"1998","unstructured":"Koya, D. & King, G. L. Protein kinase C activation and the development of diabetic complications. Diabetes 47, 859\u2013866 (1998).","journal-title":"Diabetes"},{"issue":"3","key":"49600_CR30","doi-asserted-by":"publisher","first-page":"3549","DOI":"10.1096\/fj.201801855R","volume":"33","author":"Luqing Zhao","year":"2019","unstructured":"Zhao, L. et al. Hyperglycemia promotes microvillus membrane expression of DMT1 in intestinal epithelial cells in a PKCalpha-dependent manner. FASEB journal: official publication of the Federation of American Societies for Experimental Biology, fj201801855R, https:\/\/doi.org\/10.1096\/fj.201801855R (2018).","journal-title":"The FASEB Journal"},{"key":"49600_CR31","doi-asserted-by":"publisher","first-page":"294","DOI":"10.1038\/nm834","volume":"9","author":"HP Hammes","year":"2003","unstructured":"Hammes, H. P. et al. Benfotiamine blocks three major pathways of hyperglycemic damage and prevents experimental diabetic retinopathy. Nature medicine 9, 294\u2013299, https:\/\/doi.org\/10.1038\/nm834 (2003).","journal-title":"Nature medicine"},{"key":"49600_CR32","doi-asserted-by":"publisher","first-page":"1260419","DOI":"10.1126\/science.1260419","volume":"347","author":"M Uhlen","year":"2015","unstructured":"Uhlen, M. et al. Tissue-based map of the human proteome. Science 347, 1260419, https:\/\/doi.org\/10.1126\/science.1260419 (2015).","journal-title":"Science"},{"key":"49600_CR33","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1530\/ror.0.0040151","volume":"4","author":"H Breitbart","year":"1999","unstructured":"Breitbart, H. & Naor, Z. Protein kinases in mammalian sperm capacitation and the acrosome reaction. Rev Reprod 4, 151\u2013159 (1999).","journal-title":"Rev Reprod"},{"key":"49600_CR34","doi-asserted-by":"publisher","first-page":"841615","DOI":"10.1155\/2014\/841615","volume":"2014","author":"MS Rahman","year":"2014","unstructured":"Rahman, M. S., Kwon, W. S. & Pang, M. G. Calcium influx and male fertility in the context of the sperm proteome: an update. BioMed research international 2014, 841615, https:\/\/doi.org\/10.1155\/2014\/841615 (2014).","journal-title":"BioMed research international"},{"key":"49600_CR35","doi-asserted-by":"publisher","first-page":"709","DOI":"10.1111\/j.1365-2605.2009.01013.x","volume":"33","author":"J O\u2019Neill","year":"2010","unstructured":"O\u2019Neill, J. et al. Differences in mouse models of diabetes mellitus in studies of male reproduction. Int J Androl 33, 709\u2013716, https:\/\/doi.org\/10.1111\/j.1365-2605.2009.01013.x (2010).","journal-title":"Int J Androl"},{"key":"49600_CR36","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/S1056-8727(02)00281-7","volume":"18","author":"J Miura","year":"2004","unstructured":"Miura, J. et al. AGE down-regulation of monocyte RAGE expression and its association with diabetic complications in type 1 diabetes. Journal of diabetes and its complications 18, 53\u201359, https:\/\/doi.org\/10.1016\/S1056-8727(02)00281-7 (2004).","journal-title":"Journal of diabetes and its complications"},{"key":"49600_CR37","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1161\/CIRCULATIONAHA.106.621854","volume":"114","author":"A Goldin","year":"2006","unstructured":"Goldin, A., Beckman, J. A., Schmidt, A. M. & Creager, M. A. Advanced glycation end products: sparking the development of diabetic vascular injury. Circulation 114, 597\u2013605, https:\/\/doi.org\/10.1161\/CIRCULATIONAHA.106.621854 (2006).","journal-title":"Circulation"},{"key":"49600_CR38","doi-asserted-by":"publisher","first-page":"891","DOI":"10.1002\/j.1939-4640.2003.tb03141.x","volume":"24","author":"ML Hixon","year":"2003","unstructured":"Hixon, M. L. & Boekelheide, K. Expression and localization of total Akt1 and phosphorylated Akt1 in the rat seminiferous epithelium. J Androl 24, 891\u2013898 (2003).","journal-title":"J Androl"},{"key":"49600_CR39","doi-asserted-by":"publisher","first-page":"1023","DOI":"10.1517\/14728222.2011.575360","volume":"15","author":"JA Nogueira-Machado","year":"2011","unstructured":"Nogueira-Machado, J. A., Volpe, C. M., Veloso, C. A. & Chaves, M. M. HMGB1, TLR and RAGE: a functional tripod that leads to diabetic inflammation. Expert Opin Ther Targets 15, 1023\u20131035, https:\/\/doi.org\/10.1517\/14728222.2011.575360 (2011).","journal-title":"Expert Opin Ther Targets"},{"key":"49600_CR40","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1093\/toxsci\/kfn186","volume":"106","author":"R Rogers","year":"2008","unstructured":"Rogers, R. et al. Cross-talk between the Akt and NF-kappaB signaling pathways inhibits MEHP-induced germ cell apoptosis. Toxicological sciences: an official journal of the Society of Toxicology 106, 497\u2013508, https:\/\/doi.org\/10.1093\/toxsci\/kfn186 (2008).","journal-title":"Toxicological sciences: an official journal of the Society of Toxicology"},{"key":"49600_CR41","doi-asserted-by":"publisher","first-page":"11399","DOI":"10.1073\/pnas.1001077107","volume":"107","author":"EW Wong","year":"2010","unstructured":"Wong, E. W., Mruk, D. D., Lee, W. M. & Cheng, C. Y. Regulation of blood-testis barrier dynamics by TGF-beta3 is a Cdc42-dependent protein trafficking event. Proceedings of the National Academy of Sciences of the United States of America 107, 11399\u201311404, https:\/\/doi.org\/10.1073\/pnas.1001077107 (2010).","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"49600_CR42","doi-asserted-by":"publisher","first-page":"255","DOI":"10.2147\/DMSO.S50516","volume":"7","author":"AR Gosmanov","year":"2014","unstructured":"Gosmanov, A. R., Gosmanova, E. O. & Dillard-Cannon, E. Management of adult diabetic ketoacidosis. Diabetes Metab Syndr Obes 7, 255\u2013264, https:\/\/doi.org\/10.2147\/DMSO.S50516 (2014).","journal-title":"Diabetes Metab Syndr Obes"},{"key":"49600_CR43","doi-asserted-by":"publisher","first-page":"1969","DOI":"10.1038\/nprot.2014.129","volume":"9","author":"N Rabbani","year":"2014","unstructured":"Rabbani, N. & Thornalley, P. J. Measurement of methylglyoxal by stable isotopic dilution analysis LC-MS\/MS with corroborative prediction in physiological samples. Nat Protoc 9, 1969\u20131979, https:\/\/doi.org\/10.1038\/nprot.2014.129 (2014).","journal-title":"Nat Protoc"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-019-49600-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-019-49600-4","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-019-49600-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,17]],"date-time":"2022-12-17T08:25:30Z","timestamp":1671265530000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-019-49600-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,10]]},"references-count":43,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2019,12]]}},"alternative-id":["49600"],"URL":"https:\/\/doi.org\/10.1038\/s41598-019-49600-4","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,10]]},"assertion":[{"value":"13 March 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 August 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 September 2019","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"13074"}}