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For this, rabbit polyclonal antibody with specificity to <jats:italic>C. jejuni<\/jats:italic> was first mixed with <jats:italic>C. jejuni<\/jats:italic> cells and unbound antibody was subsequently separated using a sequential process of centrifugation and then detected using an immobilized goat anti-rabbit IgG polyclonal antibody on the SPR sensor chip. This SIA-SPR method showed excellent sensitivity for <jats:italic>C<\/jats:italic>. <jats:italic>jejuni<\/jats:italic> with a limit of detection (LOD) of 131\u2009\u00b1\u20094 CFU mL<jats:sup>\u22121<\/jats:sup> and a 95% confidence interval from 122 to 140 CFU mL<jats:sup>\u22121<\/jats:sup>. The method has also high specificity. The developed method showed low cross-reactivity to bacterial pathogens such as <jats:italic>Salmonella<\/jats:italic><jats:italic>enterica<\/jats:italic> serovar Typhimurium (7.8%), <jats:italic>Listeria monocytogenes<\/jats:italic> (3.88%) and <jats:italic>Escherichia coli<\/jats:italic> (1.56%). The SIA-SPR method together with the culturing (plating) method was able to detect <jats:italic>C<\/jats:italic>. <jats:italic>jejuni<\/jats:italic> in the real chicken sample at less than 500 CFU mL<jats:sup>\u22121<\/jats:sup>, the minimum infectious dose for <jats:italic>C<\/jats:italic>. <jats:italic>jejuni<\/jats:italic> while a commercial ELISA kit was unable to detect the bacterium. Since the currently available detection tools rely on culturing methods, which take more than 48\u2009hours to detect the bacterium, the developed method in this work has the potential to be a rapid and sensitive detection method for <jats:italic>C<\/jats:italic>. <jats:italic>jejuni<\/jats:italic>.<\/jats:p>","DOI":"10.1038\/s41598-019-49672-2","type":"journal-article","created":{"date-parts":[[2019,9,20]],"date-time":"2019-09-20T10:15:48Z","timestamp":1568974548000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Subtractive inhibition assay for the detection of Campylobacter jejuni in chicken samples using surface plasmon resonance"],"prefix":"10.1038","volume":"9","author":[{"given":"Noor Azlina","family":"Masdor","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeynep","family":"Altintas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohd. 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