{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T08:16:30Z","timestamp":1793261790750,"version":"4.5.0"},"reference-count":32,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2019,9,16]],"date-time":"2019-09-16T00:00:00Z","timestamp":1568592000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2019,9,16]],"date-time":"2019-09-16T00:00:00Z","timestamp":1568592000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Surgical oncologists depend heavily on visual field acuity during cancer resection surgeries for <jats:italic>in-situ<\/jats:italic> margin assessment. Clinicians must wait up to two weeks for results from a pathology lab to confirm a post-operative diagnosis, potentially resulting in subsequent treatments. Currently, there are no clinical tools that can visualize diagnostically pertinent tissue information <jats:italic>in-situ<\/jats:italic>. Here, we present the first microscopy capable of non-contact label-free visualization of human\u00a0cellular morphology in a reflection-mode apparatus. This is possible with the recently reported imaging modality called photoacoustic remote sensing microscopy which enables non-contact detection of optical absorption contrast. By taking advantage of the 266-nanometer optical absorption peak of DNA, photoacoustic remote sensing is efficacious in recovering qualitatively similar nuclear information in comparison to that provided by the hematoxylin stain in the gold-standard hematoxylin and eosin (H&amp;E) prepared samples. A photoacoustic remote sensing system was employed utilizing a 266-nanometer pulsed excitation beam to induce photoacoustic pressures within the sample resulting in refractive index modulation of the optical absorber. A 1310-nanometer continuous-wave interrogation beam detects these perturbed regions as back reflected intensity variations due to the changes in the local optical properties. Using this technique, clinically useful histologic images of human tissue samples including breast cancer (invasive ductal carcinoma), tonsil, gastrointestinal, and pancreatic tissue images were formed. These were qualitatively comparable to standard H&amp;E prepared samples.<\/jats:p>","DOI":"10.1038\/s41598-019-49849-9","type":"journal-article","created":{"date-parts":[[2019,9,16]],"date-time":"2019-09-16T10:05:08Z","timestamp":1568628308000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["All-optical Reflection-mode Microscopic Histology of Unstained Human Tissues"],"prefix":"10.1038","volume":"9","author":[{"given":"Saad","family":"Abbasi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Le","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bazil","family":"Sonier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepak","family":"Dinakaran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilbert","family":"Bigras","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevan","family":"Bell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John R.","family":"Mackey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parsin","family":"Haji Reza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,9,16]]},"reference":[{"issue":"9","key":"49849_CR1","doi-asserted-by":"publisher","first-page":"1501","DOI":"10.1016\/j.humpath.2011.11.010","volume":"43","author":"Samip Patel","year":"2012","unstructured":"Patel, S., Smith, J. B., Kurbatova, E. & Guarner, J. Factors that impact turnaround time of surgical pathology specimens in an academic institution. Hum. Pathol., https:\/\/doi.org\/10.1016\/j.humpath.2011.11.010 (2012).","journal-title":"Human Pathology"},{"issue":"2","key":"49849_CR2","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1002\/jso.21215","volume":"99","author":"Michael S. Sabel","year":"2009","unstructured":"Sabel, M. S. et al. Residual disease after re-excision lumpectomy for close margins. J. Surg. Oncol., https:\/\/doi.org\/10.1002\/jso.21215 (2009).","journal-title":"Journal of Surgical Oncology"},{"issue":"5","key":"49849_CR3","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1001\/jama.2012.43","volume":"307","author":"Laurence E. McCahill","year":"2012","unstructured":"L.E., M. et al. Variability in reexcision following breast conservation surgery. JAMA - J. Am. Med. 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Toward wide-field high-speed photoacoustic remote sensing microscopy., https:\/\/doi.org\/10.1117\/12.2291036 (2018).","DOI":"10.1117\/12.2291036"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-019-49849-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-019-49849-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-019-49849-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,17]],"date-time":"2022-12-17T13:41:03Z","timestamp":1671284463000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-019-49849-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,16]]},"references-count":32,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2019,12]]}},"alternative-id":["49849"],"URL":"https:\/\/doi.org\/10.1038\/s41598-019-49849-9","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,16]]},"assertion":[{"value":"6 June 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 September 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 September 2019","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"D. D., K. B., J. R. M. and P. H. R. have financial interests in illumiSonics Inc. IllumiSonics partly supported this work.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"13392"}}