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Genetic diversity of <jats:italic>Plasmodium<\/jats:italic> parasites regulates transmission dynamics, disease severity and vaccine efficacy. <jats:italic>P<\/jats:italic>. <jats:italic>falciparum<\/jats:italic> chloroquine resistance transporter (<jats:italic>Pfcrt<\/jats:italic>), multidrug resistance-1 (<jats:italic>Pfmdr-1<\/jats:italic>) and kelch 13 propeller (<jats:italic>PfK-13<\/jats:italic>) genes which govern antimalarial drug resistance and three genetic diversity markers, merozoite surface protein 1 and 2 (<jats:italic>Pfmsp-1<\/jats:italic>, <jats:italic>Pfmsp-2<\/jats:italic>) and glutamate rich protein (<jats:italic>Pfglurp<\/jats:italic>) were evaluated from Tripura, Northeast India using molecular tools. In the <jats:italic>Pfcrt<\/jats:italic> gene, 87% isolates showed triple mutations at codons M74I, N75E and K76T. 12.5% isolates in <jats:italic>Pfmdr-1<\/jats:italic> gene showed mutation at N86Y. No polymorphism in <jats:italic>PfK-13 propeller<\/jats:italic> was found. Polyclonal infections were observed in 53.85% isolates and more commonly in adults (p\u2009=\u20090.0494). In the <jats:italic>Pfmsp-1<\/jats:italic> locus, the K1 allelic family was predominant (71.2%) followed by the 3D7\/IC family (69.2%) in the <jats:italic>Pfmsp-2<\/jats:italic> locus. RII region of <jats:italic>Pfglurp<\/jats:italic> exhibited nine alleles with expected heterozygosity of 0.85. The multiplicity of infection for <jats:italic>Pfmsp-1<\/jats:italic>, <jats:italic>Pfmsp-2<\/jats:italic> and <jats:italic>Pfglurp<\/jats:italic> were 1.56, 1.31 and 1.06 respectively. Overall, the study demonstrated a high level of chloroquine resistance and extensive parasite diversity in the region, necessitating regular surveillance in this population group.<\/jats:p>","DOI":"10.1038\/s41598-019-50152-w","type":"journal-article","created":{"date-parts":[[2019,9,23]],"date-time":"2019-09-23T10:02:55Z","timestamp":1569232975000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Characterization of drug resistance and genetic diversity of Plasmodium falciparum parasites from Tripura, Northeast India"],"prefix":"10.1038","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0651-2739","authenticated-orcid":false,"given":"S. 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