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We have shown that liver injury and\n                    <jats:italic>Ehrlichia<\/jats:italic>\n                    -induced sepsis occur due to dysregulated inflammation. Here, we investigated the contribution of macrophages to\n                    <jats:italic>Ehrlichia<\/jats:italic>\n                    -induced sepsis using murine models of mild and fatal ehrlichiosis. Lethally-infected mice showed accumulation of M1 macrophages (iNOS-positive) in the liver. In contrast, non-lethally infected mice showed polarization of M2 macrophages and their accumulation in peritoneum, but not in the liver. Predominance of M1 macrophages in lethally-infected mice was associated with expansion of IL-17-producing T, NK, and NKT cells. Consistent with the\n                    <jats:italic>in vivo data<\/jats:italic>\n                    , infection of bone marrow-derived macrophages (BMM) with lethal\n                    <jats:italic>Ehrlichia<\/jats:italic>\n                    polarized M0 macrophages into M1 phenotype under an mTORC1-dependent manner, while infection with non-lethal\n                    <jats:italic>Ehrlichia<\/jats:italic>\n                    polarized these cells into M2 types. This work highlights that mTORC1-mediated polarization of macrophages towards M1 phenotype may contribute to induction of pathogenic immune responses during fatal ehrlichiosis. Targeting mTORC1 pathway may provide a novel aproach for treatment of HME.\n                  <\/jats:p>","DOI":"10.1038\/s41598-019-50320-y","type":"journal-article","created":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T06:02:32Z","timestamp":1569909752000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["mTORC1-mediated polarization of M1 macrophages and their accumulation in the liver correlate with immunopathology in fatal ehrlichiosis"],"prefix":"10.1038","volume":"9","author":[{"given":"Mohamed","family":"Haloul","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8606-7454","authenticated-orcid":false,"given":"Edson R. 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