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The last decade has seen a growing incidence of Lyme disease in Canada, following the northward range expansion of <jats:italic>I. scapularis<\/jats:italic> tick populations from endemic areas in eastern United States. This may be attributable to movement of the many hosts that they parasitize, including songbirds, deer and small mammals. In this study, we wanted to test the effect of spatial, temporal and ecological variables, on blacklegged tick density and infection rates, near the northern limit of their distribution in Ontario and Quebec, Canada. We found an effect of both proportion of forested areas and distance to roads, on\u00a0density of <jats:italic>I. scapularis<\/jats:italic> ticks and prevalence\u00a0of infection by <jats:italic>B. burgdorferi<\/jats:italic>. We also found an effect of both sampling year and ordinal sampling data\u00a0on prevalence of infection by <jats:italic>B. burgdorferi<\/jats:italic>. In six adjacent sites showing evidence of reproducing <jats:italic>I. scapularis<\/jats:italic> populations, we found that forest composition and structure influenced density of <jats:italic>I. scapularis<\/jats:italic> ticks. Our results suggest that blacklegged tick density and infection rate in Canada may be influenced by a variety of factors.<\/jats:p>","DOI":"10.1038\/s41598-019-50858-x","type":"journal-article","created":{"date-parts":[[2019,11,13]],"date-time":"2019-11-13T11:04:01Z","timestamp":1573643041000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Landscape determinants of density of blacklegged ticks, vectors of Lyme disease, at the northern edge of their distribution in Canada"],"prefix":"10.1038","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3567-1213","authenticated-orcid":false,"given":"Benoit","family":"Talbot","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreea","family":"Slatculescu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles R.","family":"Thickstun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jules K.","family":"Koffi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick A.","family":"Leighton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roman","family":"McKay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manisha A.","family":"Kulkarni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,11,13]]},"reference":[{"key":"50858_CR1","doi-asserted-by":"publisher","first-page":"1093","DOI":"10.1172\/JCI21681","volume":"113","author":"AC Steere","year":"2004","unstructured":"Steere, A. 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