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High-intensity focused ultrasound (HIFU) provides a means to actuate matter from a distance in a non-destructive way. In this study, we aimed to deliver methylene blue locally into bovine articular cartilage <jats:italic>in vitro<\/jats:italic>. HIFU-treated samples (<jats:italic>n<\/jats:italic>\u2009=\u200910) were immersed in a methylene blue (MB) solution during sonication (<jats:italic>f<\/jats:italic>\u2009=\u20092.16\u2009MHz, peak-positive-pressure\u2009=\u20093.5\u2009MPa, mechanical index\u2009=\u20091.8, pulse repetition frequency\u2009=\u20093.0\u2009kHz, cycles per burst: 50, duty cycle: 7%). Adjacent control 1 tissue (<jats:italic>n<\/jats:italic>\u2009=\u200910) was first pre-treated with HIFU followed by immersion into MB; adjacent control 2 tissue (<jats:italic>n<\/jats:italic>\u2009=\u200910) was immersed in MB without ultrasound exposure. The MB content was higher (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) in HIFU-treated samples all the way to a depth of 600\u2009\u00b5m from AC surface when compared to controls. Chondrocyte viability and RNA expression levels associated with cartilage degeneration were not different in HIFU-treated samples when compared to controls (<jats:italic>p<\/jats:italic>\u2009&gt;\u20090.05). To conclude, HIFU delivers molecules into articular cartilage without major short-term concerns about safety. The method is a candidate for a future approach for managing OA.<\/jats:p>","DOI":"10.1038\/s41598-019-52012-z","type":"journal-article","created":{"date-parts":[[2019,11,4]],"date-time":"2019-11-04T11:03:13Z","timestamp":1572865393000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Localized delivery of compounds into articular cartilage by using high-intensity focused ultrasound"],"prefix":"10.1038","volume":"9","author":[{"given":"Heikki J.","family":"Nieminen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eetu","family":"Lampsij\u00e4rvi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gon\u00e7alo","family":"Barreto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3348-5759","authenticated-orcid":false,"given":"Mikko A. 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