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We investigated the relationship between HSP47 expression and tissue remodeling in CRS. We also determined the underlying molecular mechanisms of TGF-\u03b21-induced HSP47 and extracellular matrix (ECM) production in nasal fibroblasts. HSP47, \u03b1-SMA, fibronectin, and collagen type I expression levels were measured using real-time PCR, western blotting, and immunofluorescence staining. Fibroblast migration was analyzed using scratch and transwell migration assays. Contractile activity was measured with a collagen gel contraction assay. HSP47 is increased in patients with CRS without nasal polyps. TGF-\u03b21 induced HSP47 expression in nasal fibroblasts. Myofibroblast differentiation and ECM production, which are induced by TGF-\u03b21, were inhibited by <jats:italic>siHSP47<\/jats:italic>. We also confirmed that the Smad2\/3 signaling pathway is involved in TGF-\u03b21-induced HSP47 expression in nasal fibroblasts. In a functional assay, TGF-\u03b21-enhanced migration and contraction ability were inhibited by HSP47 knockout. Glucocorticoid reversed the stimulatory effects of TGF-\u03b21 on HSP47 expression and ECM production in nasal fibroblasts and <jats:italic>ex vivo<\/jats:italic> organ cultures. HSP47 expression is involved in TGF-\u03b21-induced myofibroblast differentiation and ECM production through the Smad2\/3 signaling pathway, which might contribute to tissue remodeling in chronic rhinosinusitis.<\/jats:p>","DOI":"10.1038\/s41598-019-52064-1","type":"journal-article","created":{"date-parts":[[2019,10,30]],"date-time":"2019-10-30T20:29:48Z","timestamp":1572467388000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["TGF-\u03b21-induced HSP47 regulates extracellular matrix accumulation via Smad2\/3 signaling pathways in nasal fibroblasts"],"prefix":"10.1038","volume":"9","author":[{"given":"Hae-Ji","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3221-640X","authenticated-orcid":false,"given":"Joo-Hoo","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae-Min","family":"Shin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun-Woo","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heung-Man","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7011-6071","authenticated-orcid":false,"given":"Il-Ho","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,10,29]]},"reference":[{"key":"52064_CR1","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1177\/194589240401800502","volume":"18","author":"JB Watelet","year":"2004","unstructured":"Watelet, J. 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