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Serine protease inhibitors (SPIs) have unique enzyme inhibitory activity and occupy an important position in the interaction between parasites and hosts. In order to further understand the immunoprotective effect of SPIs on <jats:italic>T. spiralis<\/jats:italic> invasion <jats:italic>in vivo<\/jats:italic>, the Kazal and Serpin type SPI of <jats:italic>T. spiralis<\/jats:italic> (TsKaSPI and TsAdSPI) were mixed with Freund\u2019s adjuvant in equal volume to immunize mice. The results showed that the expression of IgG1 and IgG2a in serum, the proliferation of spleen cells, and the expression level of cytokines were all increased. The results of flow cytometry showed that the expression of CD4+CD25+Foxp3+ Tregs, CD8+CD28\u2212 T cells, CD19+CD5+CD1d<jats:sup>hi<\/jats:sup> Bregs in spleen were also increased. Therefore, both TsKaSPI and TsAdSPI could induce strong humoral and cellular immune responses. And the results of adult reduction rate and pathological changes of intestine after adult invasion also indicated that both TsKaSPI and TsAdSPI could prevent <jats:italic>T. spiralis<\/jats:italic> from invading intestine. To explore the regulatory effects of TsKaSPI and TsAdSPI on the immune function of macrophage, the results of ELISA showed that the expression of cytokines in cell supernatant were increased. And the results of Western blot showed that both TsKaSPI and TsAdSPI could induce phosphorylation of JAK2 and STAT3 receptors, thereby affecting the signal transduction of macrophages. This experiment demonstrated that SPIs could act as effector molecules affecting the immune function of host when infected with <jats:italic>T. spiralis<\/jats:italic>.<\/jats:p>","DOI":"10.1038\/s41598-019-52624-5","type":"journal-article","created":{"date-parts":[[2019,11,19]],"date-time":"2019-11-19T11:04:13Z","timestamp":1574161453000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Regulatory effect of two Trichinella spiralis serine protease inhibitors on the host\u2019s immune system"],"prefix":"10.1038","volume":"9","author":[{"given":"Jingyun","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengcheng","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijia","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixin","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,11,19]]},"reference":[{"key":"52624_CR1","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/j.vetpar.2013.01.038","volume":"194","author":"J Cui","year":"2013","unstructured":"Cui, J. et al. 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