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This infection is re-emerging and new features related to Bordetella pathogenesis and microbiology could be relevant to defeat it. Therefore, we focused our attention on BP1253, a predicted exported protein from <jats:italic>B. pertussis<\/jats:italic> erroneously classified as lysine decarboxylase. We showed that BP1253 shares the highly conserved motif PGGxGTxxE and the key catalytic amino-acid residues with newly structurally characterized \u201cLONELY GUY\u201d (LOG) proteins. Biochemical studies have confirmed that this protein functions as a cytokinin-activating enzyme since it cleaves the N-glycosidic linkage between the base and the ribose, leading to the formation of free bases, which are the active form of plant hormones called cytokinins. Remarkably, BP1253 selectively binds monophosphate nucleotides such as AMP, GMP and CMP, showing a wider variety in binding capacity compared to other LOGs. Cytokinin production studies performed with <jats:italic>B. pertussis<\/jats:italic> have revealed 6-O-methylguanine to be the physiological product of BP1253 in agreement with the higher activity of the enzyme towards GMP. 6-O-methylguanine is likely to be responsible for the increased sensitivity of <jats:italic>B. pertussis<\/jats:italic> to oxidative stress. Although BP1253 has a primary sequence resembling the hexameric type-II LOGs, the dimeric state and the presence of specific amino-acids suggests that BP1253 can be classified as a novel type-II LOG. The discovery of a LOG along with its product 6-O-methylguanine in the human pathogen <jats:italic>B. pertussis<\/jats:italic> may lead to the discovery of unexplored functions of LOGs, broadening their role beyond plants.<\/jats:p>","DOI":"10.1038\/s41598-019-53171-9","type":"journal-article","created":{"date-parts":[[2019,11,19]],"date-time":"2019-11-19T11:04:13Z","timestamp":1574161453000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A LONELY GUY protein of Bordetella pertussis with unique features is related to oxidative stress"],"prefix":"10.1038","volume":"9","author":[{"given":"Filippo","family":"Moramarco","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfredo","family":"Pezzicoli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Salvini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosanna","family":"Leuzzi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Werner","family":"Pansegrau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrico","family":"Balducci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,11,19]]},"reference":[{"key":"53171_CR1","doi-asserted-by":"publisher","first-page":"328","DOI":"10.1086\/589753","volume":"47","author":"CD Paddock","year":"2008","unstructured":"Paddock, C. 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F.M. received a GSK graduate fellowship from the University of Bologna. E.B. and L.S. report no financial competing interests. The authors declare no non-financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"17016"}}