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Because of their persistency, bioaccumulation and potential for secondary intoxication, they have faced increasing legislative regulations. Recently, the European Union Regulation (EU) 2016\/1179 resulted in the production and application of rodenticides with nearly half dose (&lt;30 ppm) of anticoagulants. However, published data on the biological efficacy of rodenticides with decreased doses are scarce in the EU. Therefore, this work compared the efficacy of the original high-dose (50 ppm) and new low-dose (25 ppm) brodifacoum-based baits in the offspring of wild-caught house mice (<jats:italic>Mus musculus<\/jats:italic> L.). In the no-choice laboratory feeding tests, 100% animals died in all treated groups and 0% died in the control groups. The achieved time to death did not differ between the original and low-dose baits across both types of feeding trials\/regimes. The low-dose baits (25 ppm) were consequently tested under field conditions in two populations showing 95.7% and 99.8% efficacy. The obtained results highlighted the good efficacy of the new baits based on low-dose brodifacoum in non-resistant mouse populations. However, further validation is required regarding the remaining anticoagulant compounds and resistant rodent populations.<\/jats:p>","DOI":"10.1038\/s41598-019-53299-8","type":"journal-article","created":{"date-parts":[[2019,11,14]],"date-time":"2019-11-14T11:03:11Z","timestamp":1573729391000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Efficacy of rodenticide baits with decreased concentrations of brodifacoum: Validation of the impact of the new EU anticoagulant regulation"],"prefix":"10.1038","volume":"9","author":[{"given":"Marcela","family":"Frankova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vaclav","family":"Stejskal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radek","family":"Aulicky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,11,14]]},"reference":[{"key":"53299_CR1","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.jspr.2018.08.006","volume":"79","author":"T Blazic","year":"2018","unstructured":"Blazic, T. et al. Brodifacoum as a first choice rodenticide for controlling bromadiolone-resistant Mus musculus. J. Stored Products Res. 79, 29\u201333 (2018).","journal-title":"J. Stored Products Res."},{"key":"53299_CR2","doi-asserted-by":"publisher","first-page":"2555","DOI":"10.1002\/ps.5040","volume":"74","author":"XH Ma","year":"2018","unstructured":"Ma, X. H. et al. Low warfarin resistance frequency in Norway rats in two cities in China after 30 years of usage of anticoagulant rodenticides. Pest Manag. Sci. 74, 2555\u20132560 (2018).","journal-title":"Pest Manag. Sci."},{"issue":"9","key":"53299_CR3","doi-asserted-by":"publisher","first-page":"e0139191","DOI":"10.1371\/journal.pone.0139191","volume":"10","author":"Anke Geduhn","year":"2015","unstructured":"Geduhn, A. et al.Relation between intensity of biocide practice and residues of anticoagulant rodenticides in red foxes (Vulpes vulpes). 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