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We therefore explored possible defects in Ca<jats:sup>2+<\/jats:sup>-signalling, which may contribute to the pathology associated with another serpinopathy, \u03b1<jats:sub>1<\/jats:sub>-antitrypsin (AAT) deficiency. Using CHO K1 cell lines stably expressing a\u00a0wild type human AAT (MAAT) and a\u00a0disease-causing polymer-forming variant (ZAAT) and the truncated variant (NHK AAT), we measured basal intracellular free Ca<jats:sup>2+<\/jats:sup>, its responses to thapsigargin (TG), an ER Ca<jats:sup>2+<\/jats:sup>-ATPase blocker, and store-operated Ca<jats:sup>2+<\/jats:sup>-entry (SOCE). Our fura2 based Ca<jats:sup>2+<\/jats:sup> measurements detected no differences between these 3 parameters in cell lines expressing MAAT and cell lines expressing ZAAT and NHK AAT mutants. Thus, in our cell-based models of \u03b11-antitrypsin (AAT) deficiency, unlike the case for FENIB, we were unable to detect defects in calcium signalling.<\/jats:p>","DOI":"10.1038\/s41598-019-53535-1","type":"journal-article","created":{"date-parts":[[2019,11,21]],"date-time":"2019-11-21T11:02:41Z","timestamp":1574334161000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Calcium signalling in mammalian cell lines expressing wild type and mutant human \u03b11-Antitrypsin"],"prefix":"10.1038","volume":"9","author":[{"given":"Nancy T.","family":"Malintan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven D.","family":"Buckingham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A.","family":"Lomas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David B.","family":"Sattelle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,11,21]]},"reference":[{"key":"53535_CR1","doi-asserted-by":"publisher","first-page":"243s","DOI":"10.1378\/chest.110.6_Supplement.243S","volume":"110","author":"RW Carrell","year":"1996","unstructured":"Carrell, R. 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