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The aim of the present study was to investigate fresh vegetables originated from open markets located in the Xining City, the Qinghai-Tibet Plateau (QTP), P.R. China for their contamination with <jats:italic>T. gondii<\/jats:italic>. A total of 279 fresh vegetable samples were collected and analysed using real-time PCR assay targeting B1 gene and multilocus genotyping. <jats:italic>T. gondii<\/jats:italic> DNA was found in 10 (3.6%) samples tested; eight of them represented <jats:italic>T. gondii<\/jats:italic> type I and remaining two <jats:italic>T. gondii<\/jats:italic> type II. The approximate level of contamination of positive vegetables samples, estimated based on quantitative real-time PCR (qPCR), ranged between less than one and 27000\u2009<jats:italic>T. gondii<\/jats:italic> oocysts per sample, with majority not exceeding several oocysts per sample. The results of the study confirmed that <jats:italic>T. gondii<\/jats:italic> is present in vegetables offered in open markets in the Qinghai province, P.R. China; eating them unwashed and raw may therefore pose a threat to consumers. This is the first investigation describing <jats:italic>T. gondii<\/jats:italic> detection in fresh vegetables intended for consumption collected from the territory of P.R. China using sensitive molecular tools.<\/jats:p>","DOI":"10.1038\/s41598-019-54073-6","type":"journal-article","created":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T11:02:41Z","timestamp":1574766161000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["First molecular detection of Toxoplasma gondii in vegetable samples in China using qualitative, quantitative real-time PCR and multilocus genotyping"],"prefix":"10.1038","volume":"9","author":[{"given":"Anna","family":"Lass","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liqing","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioannis","family":"Kontogeorgos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueyong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuping","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panagiotis","family":"Karanis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,11,26]]},"reference":[{"key":"54073_CR1","doi-asserted-by":"publisher","first-page":"1965","DOI":"10.1016\/S0140-6736(04)16412-X","volume":"363","author":"JG Montoya","year":"2004","unstructured":"Montoya, J. 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