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We have previously reported that electroporation-mediated transuterine gene transfer of\n                    <jats:italic>Connexin30<\/jats:italic>\n                    , utilizing a monophasic pulse into\n                    <jats:italic>Connexin30<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    mouse otocysts at embryonic day 11.5, is able to prevent putative hearing deterioration. However, it is not clear whether supplementary gene transfer can rescue significant morphological changes, caused by genetic deficits. In addition, with the transuterine gene transfer technique utilized in our previous report, the survival rate of embryos and their mothers after treatment was low, which became a serious obstacle for effective\n                    <jats:italic>in vivo<\/jats:italic>\n                    experiments. Here, we set out to elucidate the feasibility of supplementation therapy in\n                    <jats:italic>Slc26a4<\/jats:italic>\n                    deficient mice, utilizing biphasic pulses, optimized by modifying pulse conditions. Modification of the biphasic pulse conditions during electroporation increased the survival rate. In addition, supplementation of the target gene cDNA into the otocysts of homozygous\n                    <jats:italic>Slc24a4<\/jats:italic>\n                    knockout mice significantly prevented enlargement of the endolymphatic space in the inner ear areas; moreover, it rescued hearing and vestibular function of mice\n                    <jats:italic>in vivo<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1038\/s41598-019-54262-3","type":"journal-article","created":{"date-parts":[[2019,11,29]],"date-time":"2019-11-29T06:02:41Z","timestamp":1575007361000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Prenatal electroporation-mediated gene transfer restores Slc26a4 knock-out mouse hearing and vestibular function"],"prefix":"10.1038","volume":"9","author":[{"given":"Hiroki","family":"Takeda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5977-0056","authenticated-orcid":false,"given":"Toru","family":"Miwa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Young","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Byung Yoon","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yorihisa","family":"Orita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryosei","family":"Minoda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,11,29]]},"reference":[{"key":"54262_CR1","unstructured":"Seewald, R. 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