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Little is known about flicker-induced changes of activity of retinal neurons themselves. We non-invasively investigated flicker-induced changes of retinal ganglion cell (RGC) function in common inbred mouse strains using the pattern electroretinogram (PERG), a sensitive measure of RGC function. Flicker was superimposed on the pattern stimulus at frequencies that did not generate measurable flicker-ERG and alter the PERG response. Transition from flicker at 101\u2009Hz (control) to flicker at 11\u2009Hz (test) at constant mean luminance induced a slow reduction of PERG amplitude to a minimum (39% loss in C57BL\/6J mice and 52% loss in DBA\/2J mice) 4\u20135\u2009minutes after 11\u2009Hz flicker onset, followed by a slow recovery to baseline over 20\u2009minutes. Results demonstrate that the magnitude and temporal dynamics of RGC response induced by flicker at 11\u2009Hz can be non-invasively assessed with PERG in the mouse. This allows investigating the functional phenotype of different mouse strains as well as pathological changes in glaucoma and optic nerve disease. The non-contact flicker-PERG method opens the possibility of combined assessment of neural and vascular response dynamics.<\/jats:p>","DOI":"10.1038\/s41598-019-54930-4","type":"journal-article","created":{"date-parts":[[2019,12,16]],"date-time":"2019-12-16T09:43:27Z","timestamp":1576489407000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Adaptation of retinal ganglion cell function during flickering light in the mouse"],"prefix":"10.1038","volume":"9","author":[{"given":"Tsung-Han","family":"Chou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathon","family":"Toft-Nielsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vittorio","family":"Porciatti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,12,5]]},"reference":[{"key":"54930_CR1","doi-asserted-by":"publisher","first-page":"1685","DOI":"10.1038\/jcbfm.2013.145","volume":"33","author":"EA Newman","year":"2013","unstructured":"Newman, E. 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J.T.N. is the producer of the PERG system used in this study. He developed hardware and software for the specific needs of this study.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"18396"}}