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Novel therapeutic agents with a higher efficiency and less side effects for the treatment of certain diseases are urgently needed. Plant defensins have an integral role in a hosts\u2019 immune system and are attractive candidates for combatting drug-resistant microorganisms. Interestingly, some of these defensins also showed great potential due to their cytotoxic activity toward cancer cells. In this study, a defensin encoding gene was isolated from five legume seeds using 3\u2032 rapid amplification of cDNA ends (3\u2032 RACE) with degenerate primers and cDNA cloning strategies. Bioinformatic tools were used for<jats:italic>in silico<\/jats:italic>identification and the characterization of new sequences. To study the functional characteristics of these unique defensins, the gene encoded for<jats:italic>Sesbania javanica<\/jats:italic>defensin, designated as javanicin, was cloned into pTXB-1 plasmid and expressed in the<jats:italic>Escherichia coli<\/jats:italic>Origami 2 (DE3) strain. Under optimized conditions, a 34-kDa javanicin-intein fusion protein was expressed and approximately 2.5\u20133.5\u2009mg\/L of soluble recombinant javanicin was successfully extracted with over 90% purity. Recombinant javanicin displayed antifungal properties against human pathogenic fungi, including resistant strains, as well as cytotoxic activities toward the human breast cancer cell lines, MCF-7 &amp; MDA-MB-231. Recombinant javanicin holds great promise as a novel therapeutic agent for further medical applications.<\/jats:p>","DOI":"10.1038\/s41598-019-55044-7","type":"journal-article","created":{"date-parts":[[2019,12,5]],"date-time":"2019-12-05T11:02:42Z","timestamp":1575543762000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["A novel recombinant javanicin with dual antifungal and anti-proliferative activities"],"prefix":"10.1038","volume":"9","author":[{"given":"Santhasiri","family":"Orrapin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amornrat","family":"Intorasoot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3696-8390","authenticated-orcid":false,"given":"Sittiruk","family":"Roytrakul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathupakorn","family":"Dechsupa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiraporn","family":"Kantapan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanika","family":"Onphat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chutima","family":"Srimek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chayada","family":"Sitthidet Tharinjaroen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Usanee","family":"Anukool","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bordin","family":"Butr-Indr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ponrut","family":"Phunpae","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sorasak","family":"Intorasoot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,12,5]]},"reference":[{"key":"55044_CR1","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1002\/jor.23656","volume":"36","author":"L Bingyun","year":"2018","unstructured":"Bingyun, L. & Thomas, J. 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