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After 1\u2009min of pellicle formation on bovine enamel slabs, 12 subjects rinsed with 8\u2009ml of the fluoride test solutions (NaF, Na<jats:sub>2<\/jats:sub>PO<jats:sub>3<\/jats:sub>F, AmF, SnF<jats:sub>2<\/jats:sub>,) with 500 ppm fluoride concentration each for 1\u2009min. Additionally, rinsing without a solution (control) and rinsing with 1563 ppm SnCl<jats:sub>2<\/jats:sub> solution took place for 1\u2009min. Afterwards, fluorescence microscopy took place to visualize bacterial adhesion and glucan formation (8\u2009h oral exposition) with DAPI and ConA and the BacLight method. TEM was performed to visualize the pellicle ultrastructure together with EDX to detect stannous ions. The rinsing solutions with pure SnF<jats:sub>2<\/jats:sub> and SnCl<jats:sub>2<\/jats:sub> reduced significantly the initial bacterial colonization (DAPI). While, NaF and Na<jats:sub>2<\/jats:sub>PO<jats:sub>3<\/jats:sub>F showed no significant effect compared to the control. There was no significant difference between AmF, SnF<jats:sub>2<\/jats:sub> and SnCl<jats:sub>2<\/jats:sub>. All tested experimental solutions showed no reducing effect on the glucan formation. Considerable alterations of the pellicle ultrastructure resulted from rinsing with the Sn-containing solutions. SnF<jats:sub>2<\/jats:sub> appears to be the most effective type of fluoride to reduce initial bacterial colonization <jats:italic>in situ<\/jats:italic>. The observed effects primarily have to be attributed to the stannous ions\u2019 content.<\/jats:p>","DOI":"10.1038\/s41598-019-55083-0","type":"journal-article","created":{"date-parts":[[2019,12,6]],"date-time":"2019-12-06T11:02:54Z","timestamp":1575630174000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Influence of pure fluorides and stannous ions on the initial bacterial colonization in situ"],"prefix":"10.1038","volume":"9","author":[{"given":"Jasmin","family":"Kirsch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Hannig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pia","family":"Winkel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Basche","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgit","family":"Leis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norbert","family":"P\u00fctz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Kensche","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Hannig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,12,6]]},"reference":[{"key":"55083_CR1","doi-asserted-by":"publisher","first-page":"528","DOI":"10.1159\/000260893","volume":"19","author":"MT Smits","year":"1985","unstructured":"Smits, M. 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