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It is increasingly recognized, that some neonicotinoid insecticides have a negative impact on non-target organisms, including important pollinators such as the European honeybee\n                    <jats:italic>Apis mellifera<\/jats:italic>\n                    . Toxicity of neonicotinoids may be enhanced through simultaneous exposure with additional pesticides, which could help explain, in part, the global decline of honeybee colonies. Here we examined whether exposure effects of the neonicotinoid thiamethoxam on bee viability are enhanced by the commonly used fungicide carbendazim and the herbicide glyphosate. We also analysed alternative splicing changes upon pesticide exposure in the honeybee. In particular, we examined transcripts of three genes: (i) the stress sensor gene\n                    <jats:italic>X box binding protein-1<\/jats:italic>\n                    (\n                    <jats:italic>Xbp1)<\/jats:italic>\n                    , (ii) the\n                    <jats:italic>Down Syndrome Cell Adhesion Molecule<\/jats:italic>\n                    (\n                    <jats:italic>Dscam)<\/jats:italic>\n                    gene and iii) the\n                    <jats:italic>embryonic lethal\/abnormal visual system<\/jats:italic>\n                    (\n                    <jats:italic>elav)<\/jats:italic>\n                    gene, which are important for neuronal function. Our results showed that acute thiamethoxam exposure is not enhanced by carbendazim, nor glyphosate. Toxicity of the compounds did not trigger stress-induced, alternative splicing in the analysed mRNAs, thereby leaving dormant a cellular response pathway to these man-made environmental perturbations.\n                  <\/jats:p>","DOI":"10.1038\/s41598-019-55534-8","type":"journal-article","created":{"date-parts":[[2019,12,16]],"date-time":"2019-12-16T06:03:32Z","timestamp":1576476212000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Acute thiamethoxam toxicity in honeybees is not enhanced by common fungicide and herbicide and lacks stress-induced changes in mRNA splicing"],"prefix":"10.1038","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1730-3097","authenticated-orcid":false,"given":"P\u00e2mela","family":"Decio","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pinar","family":"Ustaoglu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3124-9303","authenticated-orcid":false,"given":"Thaisa C.","family":"Roat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osmar","family":"Malaspina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Marc","family":"Devaud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2301-0805","authenticated-orcid":false,"given":"Reinhard","family":"St\u00f6ger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Soller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,12,16]]},"reference":[{"key":"55534_CR1","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1051\/apido\/2010018","volume":"41","author":"RM Johnson","year":"2010","unstructured":"Johnson, R. 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