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Here we studied the expression profile of gonadotropin subunit and GnRH receptor genes in rat pituitary <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> to clarify their expression profiles in the absence and continuous presence of GnRH. Culturing of pituitary cells in GnRH-free conditions downregulated <jats:italic>Fshb<\/jats:italic>, <jats:italic>Cga<\/jats:italic>, and <jats:italic>Gnrhr<\/jats:italic> expression, whereas continuous treatment with GnRH agonists upregulated <jats:italic>Cga<\/jats:italic> expression progressively and <jats:italic>Gnrhr<\/jats:italic> and <jats:italic>Fshb<\/jats:italic> expression transiently, accompanied by a prolonged blockade of <jats:italic>Fshb<\/jats:italic> but not <jats:italic>Gnrhr<\/jats:italic> expression. In contrast, <jats:italic>Lhb<\/jats:italic> expression was relatively insensitive to loss of endogenous GnRH and continuous treatment with GnRH, probably reflecting the status of Egr1 and Nr5a1 expression. Similar patterns of responses were observed <jats:italic>in vivo<\/jats:italic> after administration of a GnRH agonist. However, continuous treatment with GnRH stimulated LH secretion <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>, leading to decrease in LH cell content despite high basal <jats:italic>Lhb<\/jats:italic> expression. These data suggest that blockade of <jats:italic>Fshb<\/jats:italic> expression and depletion of the LH secretory pool are two major factors accounting for weakening of the gonadotroph secretory function during continuous GnRH treatment.<\/jats:p>","DOI":"10.1038\/s41598-019-56480-1","type":"journal-article","created":{"date-parts":[[2019,12,27]],"date-time":"2019-12-27T11:02:42Z","timestamp":1577444562000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo"],"prefix":"10.1038","volume":"9","author":[{"given":"Marija M.","family":"Janjic","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael M.","family":"Pr\u00e9vide","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0227-5895","authenticated-orcid":false,"given":"Patrick A.","family":"Fletcher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8372-5941","authenticated-orcid":false,"given":"Arthur","family":"Sherman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kosara","family":"Smiljanic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Abebe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivana","family":"Bjelobaba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanko S.","family":"Stojilkovic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,12,27]]},"reference":[{"key":"56480_CR1","doi-asserted-by":"crossref","unstructured":"McArdle, C. 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