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These ion channels are also present in cardiomyocytes, but no studies have been reported to date regarding the effects of Rb1 on cardiac sodium currents (I<jats:sub>Na<\/jats:sub>), L-type calcium currents (I<jats:sub>CaL<\/jats:sub>) and action potentials (APs). Additionally, the antiarrhythmic potential of Rb1 has not been assessed. In this study, we used a whole-cell patch clamp technique to assess the effect of Rb1 on these ion channels. The results showed that Rb1 inhibited I<jats:sub>Na<\/jats:sub> and I<jats:sub>CaL<\/jats:sub>, reduced the action potential amplitude (APA) and maximum upstroke velocity (V<jats:sub>max<\/jats:sub>), and shortened the action potential duration (APD) in a concentration-dependent manner but had no effect on the inward rectifier potassium current (I<jats:sub>K1<\/jats:sub>), delayed rectifier potassium current (I<jats:sub>K<\/jats:sub>) or resting membrane potential (RMP). We also designed a pathological model at the cellular and organ level to verify the role of Rb1. The results showed that Rb1 abolished high calcium-induced delayed afterdepolarizations (DADs), depressed the increase in intracellular calcium ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>), relieved calcium overload and protected cardiomyocytes. Rb1 can also reduce the occurrence of ventricular premature beats (VPBs) and ventricular tachycardia (VT) in ischemia-reperfusion (I-R) injury.<\/jats:p>","DOI":"10.1038\/s41598-019-57010-9","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T11:02:45Z","timestamp":1577790165000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Ginsenoside Rb1 exerts antiarrhythmic effects by inhibiting INa and ICaL in rabbit ventricular myocytes"],"prefix":"10.1038","volume":"9","author":[{"given":"Zhipei","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lv","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peipei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenzhen","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Hao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youjia","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antao","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peihua","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jihua","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,12,31]]},"reference":[{"key":"57010_CR1","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1111\/j.1527-3458.2007.00023.x","volume":"13","author":"SY Nah","year":"2007","unstructured":"Nah, S. 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