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CMV placental infection is a pre-requisite for materno-fetal transmission of virus, and fetal infection. We investigated the roles of the viral pentameric complex gH\/gL\/pUL128-pUL131A, and cellular platelet-derived growth factor receptor-\u03b1 (PDGFR\u03b1) for CMV infection in first trimester extravillous-derived (SGHPL-4) and villous-derived (HTR-8\/SVneo) trophoblast cells. Infection with four CMV clinical and laboratory strains (Merlin, TB40E, Towne, AD169), and Merlin deletion mutants of UL128-, UL130-, and UL131A-genes, showed a cell type-dependent requirement of the viral pentameric complex for infection of trophoblast cells. The viral pentameric complex was essential for infection of villous trophoblasts, but non-essential for extravillous trophoblasts. Blocking of PDGFR\u03b1 in extravillous trophoblasts, which naturally express PDGFR\u03b1, inhibited entry of pentameric complex-deficient CMV strains, but not the entry of pentameric positive CMV strains. Transient expression of PDGFR\u03b1 in villous trophoblasts, which are naturally deficient in PDGFR\u03b1, promoted the entry of CMV strains lacking gH\/gL\/pUL128-pUL131A, but had no effect on entry of pentameric positive CMV strains. These results suggest PDGFR\u03b1 is an important cell receptor for entry of CMV mutant strains lacking gH\/gL\/pUL128-pUL131A complexes in some placental cells, suggesting these entry pathways could be potential antiviral targets.<\/jats:p>","DOI":"10.1038\/s41598-020-57471-3","type":"journal-article","created":{"date-parts":[[2020,1,23]],"date-time":"2020-01-23T11:02:58Z","timestamp":1579777378000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Differential Expression of PDGF Receptor-\u03b1 in Human Placental Trophoblasts Leads to Different Entry Pathways by Human Cytomegalovirus Strains"],"prefix":"10.1038","volume":"10","author":[{"given":"Zin","family":"Naing","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart T.","family":"Hamilton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendy J.","family":"van Zuylen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gillian M.","family":"Scott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William D.","family":"Rawlinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,1,23]]},"reference":[{"key":"57471_CR1","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1002\/rmv.535","volume":"17","author":"A Kenneson","year":"2007","unstructured":"Kenneson, A. & Cannon, M. 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