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The food and pharmaceutical industries use it as a natural anti-oxygenic agent. Herein, a ~52\u2009kDa protein, which is a major part of seed proteome has been purified, biochemically characterized and structure has been determined. MALDI-ESI-MS identified peptide fragments and contig-deduced sequence suggested the protein to be homologous to 7S globulins. The crystal structure shows that protein has a bicupin fold similar to 7S globulins and the electron density for a copper and acetate ligand were observed in the C-terminal barrel domain. <jats:italic>In silico<\/jats:italic> study reveals that a tripeptide (VFK) from <jats:italic>Mc<\/jats:italic>7S possess a higher binding affinity for angiotensin converting enzyme (ACE) than already reported drug Lisinopril (LPR). The protein is a glycoprotein and highly stable under varying thermal and pH conditions due to its secondary structures. The DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) assay showed the protein to have an anti-oxygenic nature and can aid in scavenging free radical from sample. The protein can assist to enhance the nutritional and functional value of food by acting as a food antioxidant. Further, characterization of <jats:italic>Mc<\/jats:italic>7S required which might add in importance of <jats:italic>Mc<\/jats:italic>7S as antioxidant, anti-diabetic and anti-hypertensive.<\/jats:p>","DOI":"10.1038\/s41598-020-58138-9","type":"journal-article","created":{"date-parts":[[2020,1,24]],"date-time":"2020-01-24T11:03:17Z","timestamp":1579863797000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Structural characterization and in-silico analysis of Momordica charantia 7S globulin for stability and ACE inhibition"],"prefix":"10.1038","volume":"10","author":[{"given":"Pooja","family":"Kesari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3812-5939","authenticated-orcid":false,"given":"Shivendra","family":"Pratap","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Poonam","family":"Dhankhar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vikram","family":"Dalal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manisha","family":"Mishra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pradyumna Kumar","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harsh","family":"Chauhan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2128-7736","authenticated-orcid":false,"given":"Pravindra","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,1,24]]},"reference":[{"key":"58138_CR1","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/j.jep.2004.08.009","volume":"96","author":"N Beloin","year":"2005","unstructured":"Beloin, N. et al. 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