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The present meta-analysis aimed to evaluate the association between <jats:italic>methylenetetrahydrofolate reductase (MTHFR)<\/jats:italic> C677T polymorphism and risk of nonsyndromic cleft lip\/palate (NSCL\/P) in cases-control studies. The PubMed\/Medline, Scopus, Web of Science, and Cochrane Library databases were searched up to April 2019 with no restrictions. The odds ratios (ORs) and 95% confidence intervals (CIs) in all analyses were calculated by Review Manager 5.3 software. The funnel plot analysis was carried out by the Comprehensive Meta-Analysis version 2.0 software. Subgroup analysis, meta-regression, and sensitivity analysis were performed for the pooled analyses. Thirty-one studies reviewed in this meta-analysis included 4710 NSCL\/P patients and 7271 controls. There was no significant association between <jats:italic>MTHFR<\/jats:italic> C677T polymorphism and NSCL\/P susceptibility related to allelic model (OR\u2009=\u20091.04; P\u2009=\u20090.49), homozygote model (OR\u2009=\u20091.11; P\u2009=\u20090.35), heterozygote model (OR\u2009=\u20090.99; P\u2009=\u20090.91), dominant model (OR\u2009=\u20091.00; P\u2009=\u20090.96), or recessive model (OR\u2009=\u20091.08; P\u2009=\u20090.23). There was no significant association between <jats:italic>MTHFR<\/jats:italic> C677T polymorphism and NSCL\/P susceptibility based on the ethnicity or the source of cases. There was a significant linear relationship between the year of publication and log ORs for the allele model. The results of the present meta-analysis failed to show an association between <jats:italic>MTHFR<\/jats:italic> C677T polymorphism and NSCL\/P susceptibility. The subgroup analyses based on the ethnicity and the source of cases further confirmed this result.<\/jats:p>","DOI":"10.1038\/s41598-020-58357-0","type":"journal-article","created":{"date-parts":[[2020,1,30]],"date-time":"2020-01-30T11:02:57Z","timestamp":1580382177000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Methylenetetrahydrofolate reductase C677T polymorphism is not associated with the risk of nonsyndromic cleft lip\/palate: An updated meta-analysis"],"prefix":"10.1038","volume":"10","author":[{"given":"Mohammad Moslem","family":"Imani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Negin","family":"Golchin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3885-6640","authenticated-orcid":false,"given":"Mohsen","family":"Safaei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farzad","family":"Rezaei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hooshyar","family":"Abbasi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3586-3012","authenticated-orcid":false,"given":"Masoud","family":"Sadeghi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pia","family":"Lopez-Jornet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hamid Reza","family":"Mozaffari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roohollah","family":"Sharifi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,1,30]]},"reference":[{"key":"58357_CR1","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1038\/nrg2933","volume":"12","author":"MJ Dixon","year":"2011","unstructured":"Dixon, M. 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