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However, molecular details of the pathogenesis and physiology of this bacterium largely remain to be elucidated. Here we identify and characterize the c-di-GMP signalling network and assess its role in biofilm formation and surface associated motility. Bioinformatic analysis revealed eleven candidate genes for c-di-GMP metabolizing proteins (GGDEF\/EAL domain proteins) in the genome of <jats:italic>A. baumannii<\/jats:italic> strain 17978. Enzymatic activity of the encoded proteins was assessed by molecular cloning and expression in the model organisms <jats:italic>Salmonella typhimurium<\/jats:italic> and <jats:italic>Vibrio cholerae<\/jats:italic>. Ten of the eleven GGDEF\/EAL proteins altered the rdar morphotype of <jats:italic>S. typhimurium<\/jats:italic> and the rugose morphotype of <jats:italic>V. cholerae<\/jats:italic>. The over expression of three GGDEF proteins exerted a pronounced effect on colony formation of <jats:italic>A. baumannii<\/jats:italic> on Congo Red agar plates. Distinct panels of GGDEF\/EAL proteins were found to alter biofilm formation and surface associated motility of <jats:italic>A. baumannii<\/jats:italic> upon over expression. The GGDEF protein A1S_3296 appeared as a major diguanylate cyclase regulating macro-colony formation, biofilm formation and the surface associated motility. AIS_3296 promotes Csu pili mediated biofilm formation. We conclude that a functional c-di-GMP signalling network in <jats:italic>A. baumannii<\/jats:italic> regulates biofilm formation and surface associated motility of this increasingly important opportunistic bacterial pathogen.<\/jats:p>","DOI":"10.1038\/s41598-020-58522-5","type":"journal-article","created":{"date-parts":[[2020,2,6]],"date-time":"2020-02-06T11:04:48Z","timestamp":1580987088000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":66,"title":["A Cyclic-di-GMP signalling network regulates biofilm formation and surface associated motility of Acinetobacter baumannii 17978"],"prefix":"10.1038","volume":"10","author":[{"given":"Irfan","family":"Ahmad","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6619-7254","authenticated-orcid":false,"given":"Evelina","family":"Nygren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fizza","family":"Khalid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si Lhyam","family":"Myint","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2991-8072","authenticated-orcid":false,"given":"Bernt Eric","family":"Uhlin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,2,6]]},"reference":[{"key":"58522_CR1","doi-asserted-by":"publisher","first-page":"1079","DOI":"10.1086\/533452","volume":"197","author":"LB Rice","year":"2008","unstructured":"Rice, L. 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