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Although neutrophils express the cytoskeletal protein vinculin, they do not form mature focal adhesions. Here, we characterize the role of vinculin in \u03b22 integrin-dependent neutrophil adhesion, migration, mechanosensing, and recruitment. We observe that knockout of vinculin attenuates, but does not completely abrogate, neutrophil adhesion, spreading, and crawling under static conditions. However, we also found that vinculin deficiency does not affect these behaviors in the presence of forces from fluid flow. In addition, we identify a role for vinculin in mechanosensing, as vinculin-deficient neutrophils exhibit attenuated spreading on stiff, but not soft, substrates. Consistent with these findings, we observe that\n                    <jats:italic>in vivo<\/jats:italic>\n                    neutrophil recruitment into the inflamed peritoneum of mice remains intact in the absence of vinculin. Together, these data suggest that while vinculin regulates some aspects of neutrophil adhesion and spreading, it may be dispensable for \u03b22 integrin-dependent neutrophil recruitment\n                    <jats:italic>in vivo<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1038\/s41598-020-58882-y","type":"journal-article","created":{"date-parts":[[2020,2,7]],"date-time":"2020-02-07T06:03:42Z","timestamp":1581055422000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Context-Dependent Role of Vinculin in Neutrophil Adhesion, Motility and Trafficking"],"prefix":"10.1038","volume":"10","author":[{"given":"Zachary S.","family":"Wilson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hadley","family":"Witt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren","family":"Hazlett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Harman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brittany M.","family":"Neumann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Whitman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohak","family":"Patel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert S.","family":"Ross","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Franck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan S.","family":"Reichner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig T.","family":"Lefort","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,2,7]]},"reference":[{"key":"58882_CR1","first-page":"G1125","volume":"276","author":"B Walzog","year":"1999","unstructured":"Walzog, B., Scharffetter-Kochanek, K. & Gaehtgens, P. Impairment of neutrophil emigration in CD18-null mice. Am. J. Physiol. 276, G1125\u20131130 (1999).","journal-title":"Am. J. Physiol."},{"key":"58882_CR2","doi-asserted-by":"publisher","first-page":"678","DOI":"10.1038\/nri2156","volume":"7","author":"K Ley","year":"2007","unstructured":"Ley, K., Laudanna, C., Cybulsky, M. I. & Nourshargh, S. Getting to the site of inflammation: the leukocyte adhesion cascade updated. Nat. Rev. Immunol. 7, 678\u2013689 (2007).","journal-title":"Nat. Rev. Immunol."},{"key":"58882_CR3","doi-asserted-by":"publisher","first-page":"668","DOI":"10.1093\/infdis\/152.4.668","volume":"152","author":"DC Anderson","year":"1985","unstructured":"Anderson, D. C. et al. The Severe and Moderate Phenotypes of Heritable Mac-1, Lfa-1 Deficiency - Their Quantitative Definition and Relation to Leukocyte Dysfunction and Clinical-Features. J. Infect. Dis. 152, 668\u2013689 (1985).","journal-title":"J. Infect. 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