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In determination of Mw by muti-angle laser light-scattering (MALS), refractive index increment (dn\/dc) is an important parameter for accuracy. Herein, the influence of dn\/dc on the Mw of HA in stroke-physiological saline solution is investigated by MALS in this work. Additionally, the Mw variation of HA in the manufacturing process of preparations is measured. It is shown that each HA sample corresponds to a specific value of dn\/dc, which is varied from 1.38 to 1.74\u2009L\/g with the Mw increasing from 13.5 to 2840\u2009kDa in solution. It is indicated by the results from both MALS approach and viscometry that appropriate dn\/dc should be selected for Mw determination. In steam sterilization process of preparations at 121\u2009\u00b0C, the Mw and conformation of HA can be accurately and rapidly determined by MALS. 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