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So far, little is known about the factors governing its infection structure development and pathogenicity. From the culture filtrate of a<jats:italic>P. palmivora<\/jats:italic>strain isolated from papaya, we identified a secreted glycoprotein of 15\u2009kDa, designated as Ppal15kDa, using liquid chromatography tandem mass spectrometry. Two gene variants,<jats:italic>Ppal15kDaA<\/jats:italic>and<jats:italic>Ppal15kDaB<\/jats:italic>were amplified from a<jats:italic>P. palmivora<\/jats:italic>papaya isolate. Transient expression of both variants in<jats:italic>Nicotiana benthamiana<\/jats:italic>by agroinfiltration enhanced<jats:italic>P. palmivora<\/jats:italic>infection. Six<jats:italic>Ppal15kDa<\/jats:italic>mutants with diverse mutations were generated via CRISPR\/Cas9-mediated gene editing. All mutants were compromised in infectivity on<jats:italic>N. benthamiana<\/jats:italic>and papaya. Two mutants with all<jats:italic>Ppal15kDa<\/jats:italic>copies mutated almost completely lost pathogenicity. The pathogenicity of the other four containing at least one wild-type copy of<jats:italic>Ppal15kDa<\/jats:italic>was compromised at varying levels. The mutants were also affected in development as they produced smaller sporangia, shorter germ tubes, and fewer appressoria. The affected levels in development corresponded to the levels of reduction in pathogenicity, suggesting that Ppal15kDa plays an important role in normal development of<jats:italic>P. palmivora<\/jats:italic>infection structures. Consistent with its role in infection structure development and pathogenicity,<jats:italic>Ppal15kDa<\/jats:italic>was found to be highly induced during appressorium formation. In addition, Ppal15kDa homologs are broadly present in<jats:italic>Phytophthora<\/jats:italic>spp., but none were characterized. Altogether, this study identified a novel component involved in development and pathogenicity of<jats:italic>P. palmivora<\/jats:italic>and possibly other<jats:italic>Phytophthora<\/jats:italic>spp. known to contain a Ppal15kDa homolog.<\/jats:p>","DOI":"10.1038\/s41598-020-59007-1","type":"journal-article","created":{"date-parts":[[2020,2,11]],"date-time":"2020-02-11T11:02:39Z","timestamp":1581418959000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["A secreted protein of 15\u2009kDa plays an important role in Phytophthora palmivora development and pathogenicity"],"prefix":"10.1038","volume":"10","author":[{"given":"Sittiporn","family":"Pettongkhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natasha","family":"Navet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Schornack","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miaoying","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nunta","family":"Churngchow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,2,11]]},"reference":[{"key":"59007_CR1","doi-asserted-by":"publisher","first-page":"1703","DOI":"10.1126\/science.1085544","volume":"300","author":"SL Baldauf","year":"2003","unstructured":"Baldauf, S. 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