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To achieve this, three voltages (9, 12, 21\u2009V) were applied for different durations (3, 5, 10\u2009min). The shock was initiated approximately three minutes after fertilization followed by incubation in ambient temperature. After incubation, hatchability and survival rates were determined while ploidy status of the treatment fishes was confirmed in one-month-old fingerlings using the exclusive triploid range of the erythrocyte major axis previously reported for the same species (11.9\u201314.9\u2009\u03bcm) and by cytogenetic analysis of the chromosome. The results showed triploidy were achieved in 10 to 85% of the treatment groups. A consistent trend of decrease in hatchability and an increase in triploidy rate was observed with increased electroporation voltages and shock durations. The mean erythrocyte major axis length of triploid progenies (3n\u2009=\u200984) was observed to be between 11.3\u201314.6\u2009\u03bcm and was higher than the range of 7.0\u201310.5\u2009\u03bcm recorded for diploid progenies (2n\u2009=\u200956). It was concluded that electric shock can be used to induce triploidy in African catfish <jats:italic>C. gariepinus<\/jats:italic>.<\/jats:p>","DOI":"10.1038\/s41598-020-59389-2","type":"journal-article","created":{"date-parts":[[2020,2,12]],"date-time":"2020-02-12T11:03:58Z","timestamp":1581505438000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["First Report on Successful Triploidy Induction in Clarias gariepinus (Burchell, 1822) Using Electroporation"],"prefix":"10.1038","volume":"10","author":[{"given":"Victor Tosin","family":"Okomoda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lubna","family":"Aminath","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunday Abraham","family":"Oladimeji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ambok Bolong","family":"Abol-Munafi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alabi Isaiah","family":"Korede","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mhd","family":"Ikhwanuddin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joshua A.","family":"Umaru","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anuar","family":"Hassan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chukwuemeka Onwuka","family":"Martins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheriff Md.","family":"Shahreza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,2,12]]},"reference":[{"key":"59389_CR1","unstructured":"FAO, 2014. 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